Результаты исследований: Научные публикации в периодических изданиях › Обзорная статья › Рецензирование
Pipeline for acquisition of quantitative data on segmentation gene expression from confocal images. / Surkova, Svetlana; Myasnikova, Ekaterina; Janssens, Hilde; Kozlov, Konstantin N.; Samsonova, Anastasia A.; Reinitz, John; Samsonova, Maria.
в: Fly, Том 2, № 2, 01.01.2008, стр. 58-66.Результаты исследований: Научные публикации в периодических изданиях › Обзорная статья › Рецензирование
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TY - JOUR
T1 - Pipeline for acquisition of quantitative data on segmentation gene expression from confocal images
AU - Surkova, Svetlana
AU - Myasnikova, Ekaterina
AU - Janssens, Hilde
AU - Kozlov, Konstantin N.
AU - Samsonova, Anastasia A.
AU - Reinitz, John
AU - Samsonova, Maria
PY - 2008/1/1
Y1 - 2008/1/1
N2 - We describe a data pipeline developed to extract the quantitative data on segmentation gene expression from confocal images of gene expression patterns in Drosophila. The pipeline consists of five steps: image segmentation, background removal, temporal characterization of an embryo, data registration and data averaging. This pipeline was successfully applied to obtain quantitative gene expression data at cellular resolution in space and at the 6.5 minute resolution in time, as well as to construct a spatiotemporal atlas of segmentation gene expression. Each data pipeline step can be easily adapted to process a wide range of images of gene expression patterns.
AB - We describe a data pipeline developed to extract the quantitative data on segmentation gene expression from confocal images of gene expression patterns in Drosophila. The pipeline consists of five steps: image segmentation, background removal, temporal characterization of an embryo, data registration and data averaging. This pipeline was successfully applied to obtain quantitative gene expression data at cellular resolution in space and at the 6.5 minute resolution in time, as well as to construct a spatiotemporal atlas of segmentation gene expression. Each data pipeline step can be easily adapted to process a wide range of images of gene expression patterns.
KW - Background removal
KW - Confocal microscopy
KW - Gene expression
KW - Image processing
KW - Image segmentation
KW - Spatial registration
UR - http://www.scopus.com/inward/record.url?scp=58149199098&partnerID=8YFLogxK
U2 - 10.4161/fly.6060
DO - 10.4161/fly.6060
M3 - Review article
C2 - 18820476
AN - SCOPUS:58149199098
VL - 2
SP - 58
EP - 66
JO - Fly
JF - Fly
SN - 1933-6934
IS - 2
ER -
ID: 34107749