TY - JOUR

T1 - Phytochemical Analysis of Extracts from Some Russian Plants Using Deep Eutectic Solvents

AU - Бочко, Татьяна Николаевна

AU - Шишов, Андрей Юрьевич

AU - Малышко, Мария

AU - Огренич, Н.А.

PY - 2025/11/1

Y1 - 2025/11/1

N2 - Abstract: Medicinal plants are a valuable source of biologically active substances used to prevent and treat diverse diseases. The major classes of these compounds—alkaloids, glycosides, flavonoids, essential oils, tannins, polyphenols, and polysaccharides—exhibit broad pharmacological activities, including antimicrobial, anti-inflammatory, antiseptic, and antioxidant effects. Phytochemical screening of plant extracts plays a crucial role in identifying these compounds and supporting the development of new therapeutic agents. In this study, a spectrophotometric phytochemical screening was performed to evaluate alkaloids, tannins, flavonoids, phenolic compounds, steroid cardiac glycosides, and polysaccharides and the antioxidant activity of extracts from three medicinal plants from different families, milk-ripened oats (Avena sativa L.), madder root (Rubia tinctorum L.), and Scotch heather (Calluna vulgaris L.), was assessed. High-performance liquid chromatography was used to achieve a more detailed chemical profile. Both conventional solvents (methanol, ethanol, water-alcohol mixtures, water, acetonitrile) and a new class of solvents, deep eutectic solvents, were used as extractants. Ultrasound-assisted extraction proceeded at 40–80°C for 10–60 min. The optimal extraction conditions were found for the major compound groups: 80°C for 60 min with conventional solvents, 50°C for 30 min for Calluna vulgaris L. with deep eutectic solvents, and 60°C for 30 min for madder root and oats with deep eutectic solvents. The results demonstrate that deep eutectic solvents effectively extract the target bioactive substances under milder conditions, underscoring their potential as environmentally sustainable extractants in phytochemical research.

AB - Abstract: Medicinal plants are a valuable source of biologically active substances used to prevent and treat diverse diseases. The major classes of these compounds—alkaloids, glycosides, flavonoids, essential oils, tannins, polyphenols, and polysaccharides—exhibit broad pharmacological activities, including antimicrobial, anti-inflammatory, antiseptic, and antioxidant effects. Phytochemical screening of plant extracts plays a crucial role in identifying these compounds and supporting the development of new therapeutic agents. In this study, a spectrophotometric phytochemical screening was performed to evaluate alkaloids, tannins, flavonoids, phenolic compounds, steroid cardiac glycosides, and polysaccharides and the antioxidant activity of extracts from three medicinal plants from different families, milk-ripened oats (Avena sativa L.), madder root (Rubia tinctorum L.), and Scotch heather (Calluna vulgaris L.), was assessed. High-performance liquid chromatography was used to achieve a more detailed chemical profile. Both conventional solvents (methanol, ethanol, water-alcohol mixtures, water, acetonitrile) and a new class of solvents, deep eutectic solvents, were used as extractants. Ultrasound-assisted extraction proceeded at 40–80°C for 10–60 min. The optimal extraction conditions were found for the major compound groups: 80°C for 60 min with conventional solvents, 50°C for 30 min for Calluna vulgaris L. with deep eutectic solvents, and 60°C for 30 min for madder root and oats with deep eutectic solvents. The results demonstrate that deep eutectic solvents effectively extract the target bioactive substances under milder conditions, underscoring their potential as environmentally sustainable extractants in phytochemical research.

KW - Avena sativaL

KW - Calluna vulgaris

KW - Rubia tinctorumL

KW - biologically active substances

KW - chromatography

KW - deep eutectic solvents

KW - spectrophotometry

UR - https://www.mendeley.com/catalogue/fe076f08-283f-323b-944f-f92e76029ec3/

U2 - 10.1134/S1061934825700996

DO - 10.1134/S1061934825700996

M3 - Article

VL - 80

SP - 1854

EP - 1863

JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1061-9348

IS - 11

ER -