Phosphorylation and dephosphorylation of RNA polymerase III holoenzyme are modifications regulating the level of transcription in vitro

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Phosphorylation and dephosphorylation of RNA polymerase III holoenzyme are modifications regulating the level of transcription in vitro. / Nikitina, T. V.; Tishchenko, L. I.; Sedova, V. M.

в: Tsitologiya, Том 44, № 3, 01.12.2002, стр. 283-284.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{1cc5631d05e24949918dc5b03b57c0bc,

title = "Phosphorylation and dephosphorylation of RNA polymerase III holoenzyme are modifications regulating the level of transcription in vitro",

abstract = "Two subforms of RNA polymerase III - IIIa and IIIb - were identified in human placenta nuclei These subforms differed in molecular weight of one subunit, and in buoyant density in glycerol concentration gradient. Protein kinase activity, which phosphorylates at least four subunits of RNA polymerase IIIa and three subunits of RNA polymerase IIIb in vitro, was copurified with both the subforms. Protein kinase activity was inhibited by wortmannin, a specific PI3-kinase inhibitor. RNA polymerase III dephosphorylation by alkaline phosphatase in vitro decreased the transcription level on specific Alu-template. The associated protein kinase was not able to phosphorylate dephosphorylated RNA polymerase IIIa and to restore the transcription level up to the control one.",

author = "Nikitina, {T. V.} and Tishchenko, {L. I.} and Sedova, {V. M.}",

year = "2002",

month = dec,

day = "1",

language = "English",

volume = "44",

pages = "283--284",

journal = "Цитология",

issn = "0041-3771",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "3",

}

RIS

TY - JOUR

T1 - Phosphorylation and dephosphorylation of RNA polymerase III holoenzyme are modifications regulating the level of transcription in vitro

AU - Nikitina, T. V.

AU - Tishchenko, L. I.

AU - Sedova, V. M.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Two subforms of RNA polymerase III - IIIa and IIIb - were identified in human placenta nuclei These subforms differed in molecular weight of one subunit, and in buoyant density in glycerol concentration gradient. Protein kinase activity, which phosphorylates at least four subunits of RNA polymerase IIIa and three subunits of RNA polymerase IIIb in vitro, was copurified with both the subforms. Protein kinase activity was inhibited by wortmannin, a specific PI3-kinase inhibitor. RNA polymerase III dephosphorylation by alkaline phosphatase in vitro decreased the transcription level on specific Alu-template. The associated protein kinase was not able to phosphorylate dephosphorylated RNA polymerase IIIa and to restore the transcription level up to the control one.

AB - Two subforms of RNA polymerase III - IIIa and IIIb - were identified in human placenta nuclei These subforms differed in molecular weight of one subunit, and in buoyant density in glycerol concentration gradient. Protein kinase activity, which phosphorylates at least four subunits of RNA polymerase IIIa and three subunits of RNA polymerase IIIb in vitro, was copurified with both the subforms. Protein kinase activity was inhibited by wortmannin, a specific PI3-kinase inhibitor. RNA polymerase III dephosphorylation by alkaline phosphatase in vitro decreased the transcription level on specific Alu-template. The associated protein kinase was not able to phosphorylate dephosphorylated RNA polymerase IIIa and to restore the transcription level up to the control one.

UR - http://www.scopus.com/inward/record.url?scp=0036043360&partnerID=8YFLogxK

M3 - Article

C2 - 12094766

AN - SCOPUS:0036043360

VL - 44

SP - 283

EP - 284

JO - Цитология

JF - Цитология

SN - 0041-3771

IS - 3

ER -

ID: 53111040