Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Phosphatidic acids mediate transport of Ca 2+ and H + through plant cell membranes. / Medvedev, Sergei; Voronina, Olga; Tankelyun, Olga; Bilova, Tatiana; Suslov, Dmitry; Bankin, Mikhail; Mackievic, Viera; Makavitskaya, Maryia; Shishova, Maria; Martinec, Jan; Smolikova, Galina; Sharova, Elena; Demidchik, Vadim.
в: Functional Plant Biology, Том 46, № 6, 2019, стр. 533-542.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Phosphatidic acids mediate transport of Ca 2+ and H + through plant cell membranes
AU - Medvedev, Sergei
AU - Voronina, Olga
AU - Tankelyun, Olga
AU - Bilova, Tatiana
AU - Suslov, Dmitry
AU - Bankin, Mikhail
AU - Mackievic, Viera
AU - Makavitskaya, Maryia
AU - Shishova, Maria
AU - Martinec, Jan
AU - Smolikova, Galina
AU - Sharova, Elena
AU - Demidchik, Vadim
PY - 2019
Y1 - 2019
N2 - Phosphatidic acids (PAs) are a key intermediate in phospholipid biosynthesis, and a central element in numerous signalling pathways. Functions of PAs are related to their fundamental role in molecular interactions within cell membranes modifying membrane bending, budding, fission and fusion. Here we tested the hypothesis that PAs are capable of direct transport of ions across bio-membranes. We have demonstrated that PAs added to the maize plasma membrane vesicles induced ionophore-like transmembrane transport of Ca 2+ , H + and Mg 2+ . PA-induced Ca 2+ fluxes increased with an increasing PAs acyl chain unsaturation. For all the PAs analysed, the effect on Ca 2+ permeability increased with increasing pH (pH 8.0 > pH 7.2 > pH 6.0). The PA-induced Ca 2+ , Mg 2+ and H + permeability was also more pronounced in the endomembrane vesicles as compared with the plasma membrane vesicles. Addition of PA to protoplasts from Arabidopsis thaliana (L.) Heynh. roots constitutively expressing aequorin triggered elevation of the cytosolic Ca 2+ activity, indicating that the observed PA-dependent Ca 2+ transport occurs in intact plants.
AB - Phosphatidic acids (PAs) are a key intermediate in phospholipid biosynthesis, and a central element in numerous signalling pathways. Functions of PAs are related to their fundamental role in molecular interactions within cell membranes modifying membrane bending, budding, fission and fusion. Here we tested the hypothesis that PAs are capable of direct transport of ions across bio-membranes. We have demonstrated that PAs added to the maize plasma membrane vesicles induced ionophore-like transmembrane transport of Ca 2+ , H + and Mg 2+ . PA-induced Ca 2+ fluxes increased with an increasing PAs acyl chain unsaturation. For all the PAs analysed, the effect on Ca 2+ permeability increased with increasing pH (pH 8.0 > pH 7.2 > pH 6.0). The PA-induced Ca 2+ , Mg 2+ and H + permeability was also more pronounced in the endomembrane vesicles as compared with the plasma membrane vesicles. Addition of PA to protoplasts from Arabidopsis thaliana (L.) Heynh. roots constitutively expressing aequorin triggered elevation of the cytosolic Ca 2+ activity, indicating that the observed PA-dependent Ca 2+ transport occurs in intact plants.
KW - Ca signaling
KW - Ca transport
KW - H transport
KW - membrane vesicles
KW - phosphatidic acids
KW - protoplasts
KW - Ca 2+ transport
KW - H + transport
KW - Ca 2+ signaling
KW - Ca2+ signaling
KW - PHOSPHOLIPASE-D
KW - CHLORTETRACYCLINE
KW - ION
KW - MECHANISMS
KW - PLASMA-MEMBRANE
KW - MAGNESIUM
KW - DIVALENT-CATIONS
KW - CALCIUM
KW - STRESS
KW - H+ transport
KW - Ca2+ transport
KW - NONSELECTIVE CATION CHANNELS
UR - http://www.scopus.com/inward/record.url?scp=85062329766&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/phosphatidic-acids-mediate-transport-ca-2-h-through-plant-cell-membranes
U2 - 10.1071/FP18242
DO - 10.1071/FP18242
M3 - Article
AN - SCOPUS:85062329766
VL - 46
SP - 533
EP - 542
JO - Functional Plant Biology
JF - Functional Plant Biology
SN - 1445-4408
IS - 6
ER -
ID: 39906183