Phenotypic expression of epigenetic determinant [ISP +] in Saccharomyces cerevisiae depends on the combination of sup35 and sup45 mutations

Standard

Phenotypic expression of epigenetic determinant [ISP ⁺] in Saccharomyces cerevisiae depends on the combination of sup35 and sup45 mutations. / Aksenova, A. Yu; Volkov, K. V.; Rovinsky, N. S.; Svitin, A. V.; Mironova, L. N.

в: Molecular Biology, Том 40, № 5, 10.2006, стр. 758-763.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{97e5a6381c1c424b8690caec78e9ee85,

title = "Phenotypic expression of epigenetic determinant [ISP +] in Saccharomyces cerevisiae depends on the combination of sup35 and sup45 mutations",

abstract = "Translation fidelity in Saccharomyces yeasts is determined by genetic and epigenetic (prion) factors. A study was made of S. cerevisiae strains containing the nonchromosomal determinant [ISP +], described earlier. Some of its properties suggest that [ISP +] is a prion. [ISP +] is expressed phenotypically as an antisuppressor of two sup35 mutations and can be cured with guanidine chloride (GuHCl). It was shown that sup35 mutants containing [ISP +] carried additional sup45 mutations. These mutations caused amino acid substitutions in different regions of translation termination factor eRF1, encoded by SUP45. Strains bearing the sup35-25 mutation contained the sup45 mutation that caused amino acid substitution at position 400 of eRF1; strains bearing sup35-10 contained the mutation that altered eRF1 at position 75. Thus, the antisuppressor phenotype of the [ISP +] strains proved to depend on the interaction of sup35 and sup45 mutations, as well as on the GuHCl-curable epigenetic determinant.",

keywords = "Epigenetic inheritance, SUP35, SUP45, Translation fidelity, Yeast prions",

author = "Aksenova, {A. Yu} and Volkov, {K. V.} and Rovinsky, {N. S.} and Svitin, {A. V.} and Mironova, {L. N.}",

note = "Funding Information: This work was supported by the Russian Foundation for Basic Research (project no. 05-04-48703) and a joint grant from CRDF and the Ministry of Education and Science of the Russian Federation (ST-012-0).",

year = "2006",

month = oct,

doi = "10.1134/S0026893306050104",

language = "English",

volume = "40",

pages = "758--763",

journal = "Molecular Biology",

issn = "0026-8933",

publisher = "Pleiades Publishing",

number = "5",

}

RIS

TY - JOUR

T1 - Phenotypic expression of epigenetic determinant [ISP +] in Saccharomyces cerevisiae depends on the combination of sup35 and sup45 mutations

AU - Aksenova, A. Yu

AU - Volkov, K. V.

AU - Rovinsky, N. S.

AU - Svitin, A. V.

AU - Mironova, L. N.

N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research (project no. 05-04-48703) and a joint grant from CRDF and the Ministry of Education and Science of the Russian Federation (ST-012-0).

PY - 2006/10

Y1 - 2006/10

N2 - Translation fidelity in Saccharomyces yeasts is determined by genetic and epigenetic (prion) factors. A study was made of S. cerevisiae strains containing the nonchromosomal determinant [ISP +], described earlier. Some of its properties suggest that [ISP +] is a prion. [ISP +] is expressed phenotypically as an antisuppressor of two sup35 mutations and can be cured with guanidine chloride (GuHCl). It was shown that sup35 mutants containing [ISP +] carried additional sup45 mutations. These mutations caused amino acid substitutions in different regions of translation termination factor eRF1, encoded by SUP45. Strains bearing the sup35-25 mutation contained the sup45 mutation that caused amino acid substitution at position 400 of eRF1; strains bearing sup35-10 contained the mutation that altered eRF1 at position 75. Thus, the antisuppressor phenotype of the [ISP +] strains proved to depend on the interaction of sup35 and sup45 mutations, as well as on the GuHCl-curable epigenetic determinant.

AB - Translation fidelity in Saccharomyces yeasts is determined by genetic and epigenetic (prion) factors. A study was made of S. cerevisiae strains containing the nonchromosomal determinant [ISP +], described earlier. Some of its properties suggest that [ISP +] is a prion. [ISP +] is expressed phenotypically as an antisuppressor of two sup35 mutations and can be cured with guanidine chloride (GuHCl). It was shown that sup35 mutants containing [ISP +] carried additional sup45 mutations. These mutations caused amino acid substitutions in different regions of translation termination factor eRF1, encoded by SUP45. Strains bearing the sup35-25 mutation contained the sup45 mutation that caused amino acid substitution at position 400 of eRF1; strains bearing sup35-10 contained the mutation that altered eRF1 at position 75. Thus, the antisuppressor phenotype of the [ISP +] strains proved to depend on the interaction of sup35 and sup45 mutations, as well as on the GuHCl-curable epigenetic determinant.

KW - Epigenetic inheritance

KW - SUP35

KW - SUP45

KW - Translation fidelity

KW - Yeast prions

UR - http://www.scopus.com/inward/record.url?scp=33749867484&partnerID=8YFLogxK

U2 - 10.1134/S0026893306050104

DO - 10.1134/S0026893306050104

M3 - Article

AN - SCOPUS:33749867484

VL - 40

SP - 758

EP - 763

JO - Molecular Biology

JF - Molecular Biology

SN - 0026-8933

IS - 5

ER -

ID: 89191542