TY - JOUR

T1 - Peculiarities of tissue imaging by SERS spectroscopy and fluorescence tomography using gold nanolabels

AU - Деменьшин, Андрей Игоревич

AU - ИСТОМИНА , М.С.

AU - Медведева, Лидия Николаевна

AU - Соловьева, Елена Викторовна

N1 - A.I. Demenshin, M.S. Istomina, L.N. Medvedeva, E.V. Solovyeva, Peculiarities of tissue imaging by SERS spectroscopy and fluorescence tomography using gold nanolabels, Journal of Biophotonics 2025 https://doi.org/10.1002/jbio.202500033

PY - 2025/11/1

Y1 - 2025/11/1

N2 - Multimodal imaging agents derived from plasmonic nanoparticles have great potential for medical diagnostics. To date, dual-mode Raman-fluorescence gold-based nanolabels have remained outside conformed in vivo studies. To overcome this lack, gold nanorods coated by a polymer shell with incorporated cyanine 7 and a bimodal optical response were prepared. The study of nanolabels imaging capabilities was performed after their intravenous administration to laboratory mice. Natural biodistribution of the labels was obtained from SERS spectra of tissue sections, fluorescence images of organs, and gold concentrations determined in the digested tissues by ICP-AES. The comparison of distribution diagrams obtained from three techniques revealed that fluorescence tomography gives the underestimated labels content in the spleen, arising as a result of emission self-quenching. The consistency of SERS and ICP-AES results opens up the opportunity to use SERS as a complementary technique to assess semi-quantitative gold nanoparticle distribution in the tissues.

AB - Multimodal imaging agents derived from plasmonic nanoparticles have great potential for medical diagnostics. To date, dual-mode Raman-fluorescence gold-based nanolabels have remained outside conformed in vivo studies. To overcome this lack, gold nanorods coated by a polymer shell with incorporated cyanine 7 and a bimodal optical response were prepared. The study of nanolabels imaging capabilities was performed after their intravenous administration to laboratory mice. Natural biodistribution of the labels was obtained from SERS spectra of tissue sections, fluorescence images of organs, and gold concentrations determined in the digested tissues by ICP-AES. The comparison of distribution diagrams obtained from three techniques revealed that fluorescence tomography gives the underestimated labels content in the spleen, arising as a result of emission self-quenching. The consistency of SERS and ICP-AES results opens up the opportunity to use SERS as a complementary technique to assess semi-quantitative gold nanoparticle distribution in the tissues.

KW - biodistribution

KW - bioimaging

KW - cyanine 7

KW - gold nanoparticle

KW - surface-enhanced Raman scattering

KW - tissue

UR - https://www.mendeley.com/catalogue/0eb72f1e-c91e-377b-a783-2e8950c359ce/

U2 - 10.1002/jbio.202500033

DO - 10.1002/jbio.202500033

M3 - Article

VL - 18

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 11

M1 - e202500033

ER -