TY - CHAP

T1 - Participation of catecholamines H2S and No in Neurotransmission, Neuromodulation and regulation of adult neurogenesis in carp brain

AU - Pushchina, E. V.

AU - Obukhov, D. K.

AU - Varaksin, A. A.

PY - 2015

Y1 - 2015

N2 - The chapter considers the overall organization of the main parts ofbrain in cyprinoid fish. It is described general cytoarchitectonical acpectsof location, elements of neural structure and the system of relations in themost important centers of brainstem, and the forebrain - as the highestintegrative center of the fish brain. It is presented a new data about adultneurogenesis and neurochemical (mediator) architectonics of the carpbrain. It is described the some zones of neurogenesis in the adult carpbrain, comparative data about immunolocalization of hydrogen sulphideproducing enzyme the cystathionine β-synthase, NADPH-diaphorase andtyrosine hydroxylase in the different regions of carp brain. It is discussedthe involvement of these neurotransmitters and gaseous intermediators in the regulation of neurotransmission in the different brain centers andprocesses of adult neurogenesis.Catecholaminergic (CA-ergic) systems in the brainstem of carp werestudied using immunohistochemical labeling of tyrosine hydroxylase.The peculiarities of localization of medullary neurons, morphology of thedendrites, and trajectories of the axon projections in the medulla of thecarp allow us to differentiate three groups of neurons, namelyinterfascicular cells, units related to the lobus vagus, and cells localizedwithin the area postrema. In the periventricular region of this cerebralzone, we found phenotypically immature forms of the TH-ip cells. Wehypothesize that, in carp, dopamine functions as an inductor ofdevelopment (morphogenetic factor) and is involved in adultneurogenesis in matrix zones of the brain.H2S-producing cells and fibers are located in all parts of the braincarp and may participate in the modulation other neurotransmittersystems of the brain. NADPH-d-producing cells have been identified indifferent parts of the brain: habenular nuclei, medial hypothalamus,isthmus, reticular formation and ventral column of spinal cord. Most ofthese nuclei in the brain cyprinoid fish are cholinergic centers. Thus, incarp brain nitric oxide can be considered as a modulator of cholinergicneurotransmission. In the carp brain have been found populations H2Sproducing cells in the areas of primary and secondary neurogenesis. H2Sproducing cells have been identified in the periventricular area ofdiencephalon, medulla oblongata and granular eminentia of cerebellum.We believe that H2S may participate in the processes regulation of adultneurogenesis, cells migration and differentiation in these areas of thebrain. Among all NO-producing cells in the adult carp brain wasdiscovered population of NADPH-d positive cells, which had a high levelof enzyme activity and located on the dorsal surfaces of telencephalon.This zone is a border of embryonic eversion, where activity ofproliferative nuclear antigen was identified. Radial-oriented fibers andcells with a high activity of NADPH-d have been revealed in medialthalamus, marginal layer of optical tectum and external wall of brainstem.NADPH-d positive cells in these areas had a high level of enzymeactivity, large nucleus located in central part of cells and high value ofnuclear-cytoplasmic ratio. We believe that NO-producing cells are alsoinvolved in adult neurogenesis, because NADPH-d positive cells arelocated on the territory of secondary neurogenesis zones. However, H2Sproducing and NO-producing populations of cells in the carp brain havedifferent spatial localizations. Thus, we believe that during the adultneurogenesis in carp brain may be involved various signaltransductorsystems.

AB - The chapter considers the overall organization of the main parts ofbrain in cyprinoid fish. It is described general cytoarchitectonical acpectsof location, elements of neural structure and the system of relations in themost important centers of brainstem, and the forebrain - as the highestintegrative center of the fish brain. It is presented a new data about adultneurogenesis and neurochemical (mediator) architectonics of the carpbrain. It is described the some zones of neurogenesis in the adult carpbrain, comparative data about immunolocalization of hydrogen sulphideproducing enzyme the cystathionine β-synthase, NADPH-diaphorase andtyrosine hydroxylase in the different regions of carp brain. It is discussedthe involvement of these neurotransmitters and gaseous intermediators in the regulation of neurotransmission in the different brain centers andprocesses of adult neurogenesis.Catecholaminergic (CA-ergic) systems in the brainstem of carp werestudied using immunohistochemical labeling of tyrosine hydroxylase.The peculiarities of localization of medullary neurons, morphology of thedendrites, and trajectories of the axon projections in the medulla of thecarp allow us to differentiate three groups of neurons, namelyinterfascicular cells, units related to the lobus vagus, and cells localizedwithin the area postrema. In the periventricular region of this cerebralzone, we found phenotypically immature forms of the TH-ip cells. Wehypothesize that, in carp, dopamine functions as an inductor ofdevelopment (morphogenetic factor) and is involved in adultneurogenesis in matrix zones of the brain.H2S-producing cells and fibers are located in all parts of the braincarp and may participate in the modulation other neurotransmittersystems of the brain. NADPH-d-producing cells have been identified indifferent parts of the brain: habenular nuclei, medial hypothalamus,isthmus, reticular formation and ventral column of spinal cord. Most ofthese nuclei in the brain cyprinoid fish are cholinergic centers. Thus, incarp brain nitric oxide can be considered as a modulator of cholinergicneurotransmission. In the carp brain have been found populations H2Sproducing cells in the areas of primary and secondary neurogenesis. H2Sproducing cells have been identified in the periventricular area ofdiencephalon, medulla oblongata and granular eminentia of cerebellum.We believe that H2S may participate in the processes regulation of adultneurogenesis, cells migration and differentiation in these areas of thebrain. Among all NO-producing cells in the adult carp brain wasdiscovered population of NADPH-d positive cells, which had a high levelof enzyme activity and located on the dorsal surfaces of telencephalon.This zone is a border of embryonic eversion, where activity ofproliferative nuclear antigen was identified. Radial-oriented fibers andcells with a high activity of NADPH-d have been revealed in medialthalamus, marginal layer of optical tectum and external wall of brainstem.NADPH-d positive cells in these areas had a high level of enzymeactivity, large nucleus located in central part of cells and high value ofnuclear-cytoplasmic ratio. We believe that NO-producing cells are alsoinvolved in adult neurogenesis, because NADPH-d positive cells arelocated on the territory of secondary neurogenesis zones. However, H2Sproducing and NO-producing populations of cells in the carp brain havedifferent spatial localizations. Thus, we believe that during the adultneurogenesis in carp brain may be involved various signaltransductorsystems.

KW - взрослый нейрогенез, цнс, позвоночные животные. гистохимия

M3 - Chapter

SN - 978-1-63463-130-3

SP - 135

EP - 192

BT - Carp and catfich.Biology, bechavior and conservation strategies

A2 - Regan, Bradley

PB - Nova Science Publishers, Inc.

CY - New York

ER -