Packaging of Subchromosomal-Size DNA Molecules in Chromatin Bodies in the Ciliate Macronucleus

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Packaging of Subchromosomal-Size DNA Molecules in Chromatin Bodies in the Ciliate Macronucleus. / Leonova, O. G.; Potekhin, A. A.; Nekrasova, I. V.; Karajan, B. P.; Syomin, B. V.; Prassolov, V. S.; Popenko, V. I.

в: Molecular Biology, Том 55, № 6, 11.2021, стр. 899-909.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Author

Leonova, O. G. ; Potekhin, A. A. ; Nekrasova, I. V. ; Karajan, B. P. ; Syomin, B. V. ; Prassolov, V. S. ; Popenko, V. I. / Packaging of Subchromosomal-Size DNA Molecules in Chromatin Bodies in the Ciliate Macronucleus. в: Molecular Biology. 2021 ; Том 55, № 6. стр. 899-909.

BibTeX

@article{58ce51ef178d4b95b966754268f02dfe,

title = "Packaging of Subchromosomal-Size DNA Molecules in Chromatin Bodies in the Ciliate Macronucleus",

abstract = "Abstract: A fundamental difference between somatic nuclei (macronuclei) of ciliates and cell nuclei of higher eukaryotes is that the macronuclear genome is a huge number (up to tens or hundreds of thousands) of gene-sized (0.5–25 kb) or subchromosomal (up to 2000 kb) minichromosomes. Electron microscopy shows that macronuclear chromatin usually looks like chromatin bodies or fibrils 200–300 nm thick in the interphase. However, the question of how many DNA molecules are contained in an individual chromatin body remains open. The organization of chromatin in macronuclei was studied in the ciliates Didinium nasutum and three Paramecium sp., which differ in pulsed-field gel electrophoresis (PFGE) karyotype, and compared with the model of topologically associated domains (TADs) of higher eukaryotic nuclei. PFGE showed that the sizes of macronuclear DNAs ranged from 50 to 1700 kb, while the majority of the molecules were less than 500 kb in length. A comparative quantitative analysis of the PFGE and electron microscopic data showed that each chromatin body contained one minichromosome in P. multimicronucleatum in the logarithmic growth phase, while bodies in the D. nasutum macronucleus contained two or more DNA molecules each. Chromatin bodies aggregated during starvation, when activity of the macronuclei decreased, leading to an increase of chromatin body size or the formation of 200- to 300-nm fibrils of several chromatin bodies. A model was proposed to explain the formation of such structures. In terms of topological characteristics, macronuclear chromatin bodies with subchromosomal DNA molecules were found to correspond to higher eukaryotic TADs.",

keywords = "chromatin, ciliates, electron microscopy, macronucleus, pulsed-field gel electrophoresis, topologically associated domains",

author = "Leonova, {O. G.} and Potekhin, {A. A.} and Nekrasova, {I. V.} and Karajan, {B. P.} and Syomin, {B. V.} and Prassolov, {V. S.} and Popenko, {V. I.}",

note = "Publisher Copyright: {\textcopyright} 2021, Pleiades Publishing, Inc.",

year = "2021",

month = nov,

doi = "10.1134/S0026893321050083",

language = "English",

volume = "55",

pages = "899--909",

journal = "Molecular Biology",

issn = "0026-8933",

publisher = "Pleiades Publishing",

number = "6",

}

RIS

TY - JOUR

T1 - Packaging of Subchromosomal-Size DNA Molecules in Chromatin Bodies in the Ciliate Macronucleus

AU - Leonova, O. G.

AU - Potekhin, A. A.

AU - Nekrasova, I. V.

AU - Karajan, B. P.

AU - Syomin, B. V.

AU - Prassolov, V. S.

AU - Popenko, V. I.

PY - 2021/11

Y1 - 2021/11

N2 - Abstract: A fundamental difference between somatic nuclei (macronuclei) of ciliates and cell nuclei of higher eukaryotes is that the macronuclear genome is a huge number (up to tens or hundreds of thousands) of gene-sized (0.5–25 kb) or subchromosomal (up to 2000 kb) minichromosomes. Electron microscopy shows that macronuclear chromatin usually looks like chromatin bodies or fibrils 200–300 nm thick in the interphase. However, the question of how many DNA molecules are contained in an individual chromatin body remains open. The organization of chromatin in macronuclei was studied in the ciliates Didinium nasutum and three Paramecium sp., which differ in pulsed-field gel electrophoresis (PFGE) karyotype, and compared with the model of topologically associated domains (TADs) of higher eukaryotic nuclei. PFGE showed that the sizes of macronuclear DNAs ranged from 50 to 1700 kb, while the majority of the molecules were less than 500 kb in length. A comparative quantitative analysis of the PFGE and electron microscopic data showed that each chromatin body contained one minichromosome in P. multimicronucleatum in the logarithmic growth phase, while bodies in the D. nasutum macronucleus contained two or more DNA molecules each. Chromatin bodies aggregated during starvation, when activity of the macronuclei decreased, leading to an increase of chromatin body size or the formation of 200- to 300-nm fibrils of several chromatin bodies. A model was proposed to explain the formation of such structures. In terms of topological characteristics, macronuclear chromatin bodies with subchromosomal DNA molecules were found to correspond to higher eukaryotic TADs.

AB - Abstract: A fundamental difference between somatic nuclei (macronuclei) of ciliates and cell nuclei of higher eukaryotes is that the macronuclear genome is a huge number (up to tens or hundreds of thousands) of gene-sized (0.5–25 kb) or subchromosomal (up to 2000 kb) minichromosomes. Electron microscopy shows that macronuclear chromatin usually looks like chromatin bodies or fibrils 200–300 nm thick in the interphase. However, the question of how many DNA molecules are contained in an individual chromatin body remains open. The organization of chromatin in macronuclei was studied in the ciliates Didinium nasutum and three Paramecium sp., which differ in pulsed-field gel electrophoresis (PFGE) karyotype, and compared with the model of topologically associated domains (TADs) of higher eukaryotic nuclei. PFGE showed that the sizes of macronuclear DNAs ranged from 50 to 1700 kb, while the majority of the molecules were less than 500 kb in length. A comparative quantitative analysis of the PFGE and electron microscopic data showed that each chromatin body contained one minichromosome in P. multimicronucleatum in the logarithmic growth phase, while bodies in the D. nasutum macronucleus contained two or more DNA molecules each. Chromatin bodies aggregated during starvation, when activity of the macronuclei decreased, leading to an increase of chromatin body size or the formation of 200- to 300-nm fibrils of several chromatin bodies. A model was proposed to explain the formation of such structures. In terms of topological characteristics, macronuclear chromatin bodies with subchromosomal DNA molecules were found to correspond to higher eukaryotic TADs.

KW - chromatin

KW - ciliates

KW - electron microscopy

KW - macronucleus

KW - pulsed-field gel electrophoresis

KW - topologically associated domains

UR - http://www.scopus.com/inward/record.url?scp=85121450785&partnerID=8YFLogxK

U2 - 10.1134/S0026893321050083

DO - 10.1134/S0026893321050083

M3 - Article

AN - SCOPUS:85121450785

VL - 55

SP - 899

EP - 909

JO - Molecular Biology

JF - Molecular Biology

SN - 0026-8933

IS - 6

ER -

ID: 91025720