TY - JOUR

T1 - Overproduction of Sch9 leads to its aggregation and cell elongation in Saccharomyces cerevisiae

AU - Drozdova, Polina

AU - Lipaeva, Polina

AU - Рогоза, Татьяна

AU - Zhouravleva, Galina

AU - Bondarev, Stanislav

PY - 2018/3/1

Y1 - 2018/3/1

N2 - The Sch9 kinase of Saccharomyces cerevisiae is one of the major TOR pathway effectors and regulates diverse processes in the cell. Sch9 belongs to the AGC kinase family. In human, amplification of AGC kinase genes is connected with cancer. However, not much is known about the effects of Sch9 overproduction in yeast cells. To fill this gap, we developed a model system to monitor subcellular location and aggregation state of overproduced Sch9 or its regions fused to a fluorescent protein. With this system, we showed that Sch9-YFP forms detergent-resistant aggregates, and multiple protein regions are responsible for this. This finding corroborated the fact that Sch9-YFP is visualized as various fluorescent foci. In addition, we found that Sch9 overproduction caused cell elongation, and this effect was determined by its C-terminal region containing kinase domains. The constructs we present can be exploited to create superior yeast-based model systems to study processes behind kinase overproduction in cancers.

AB - The Sch9 kinase of Saccharomyces cerevisiae is one of the major TOR pathway effectors and regulates diverse processes in the cell. Sch9 belongs to the AGC kinase family. In human, amplification of AGC kinase genes is connected with cancer. However, not much is known about the effects of Sch9 overproduction in yeast cells. To fill this gap, we developed a model system to monitor subcellular location and aggregation state of overproduced Sch9 or its regions fused to a fluorescent protein. With this system, we showed that Sch9-YFP forms detergent-resistant aggregates, and multiple protein regions are responsible for this. This finding corroborated the fact that Sch9-YFP is visualized as various fluorescent foci. In addition, we found that Sch9 overproduction caused cell elongation, and this effect was determined by its C-terminal region containing kinase domains. The constructs we present can be exploited to create superior yeast-based model systems to study processes behind kinase overproduction in cancers.

KW - Gene Expression Regulation, Fungal

KW - Protein Aggregates

KW - Protein Domains

KW - Protein-Serine-Threonine Kinases/chemistry

KW - Saccharomyces cerevisiae/metabolism

KW - Saccharomyces cerevisiae Proteins/chemistry

KW - MULTIPLE-MYELOMA

KW - TARGET

KW - MECHANISM

KW - PRIONS

KW - SHUTTLE VECTORS

KW - PROTEIN-KINASES

KW - BREAST-CANCER

KW - YEAST

KW - GROWTH

KW - STRESS

UR - http://www.scopus.com/inward/record.url?scp=85042794021&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/overproduction-sch9-leads-aggregation-cell-elongation-saccharomyces-cerevisiae

U2 - 10.1371/journal.pone.0193726

DO - 10.1371/journal.pone.0193726

M3 - Article

C2 - 29494682

VL - 13

SP - e0193726

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 3

M1 - e0193726

ER -