TY - JOUR

T1 - Osmotic stress is accompanied by protein glycation in Arabidopsis thaliana

AU - Paudel, Gagan

AU - Bilova, Tatiana

AU - Schmidt, Rico

AU - Greifenhagen, Uta

AU - Berger, Robert

AU - Tarakhovskaya, Elena

AU - Stöckhardt, Stefanie

AU - Balcke, Gerd Ulrich

AU - Humbeck, Klaus

AU - Brandt, Wolfgang

AU - Sinz, Andrea

AU - Vogt, Thomas

AU - Birkemeyer, Claudia

AU - Wessjohann, Ludger

AU - Frolov, Andrej

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Among the environmental alterations accompanying oncoming climate changes, drought is the most important factor influencing crop plant productivity. In plants, water deficit ultimately results in the development of oxidative stress and accumulation of osmolytes (e.g. amino acids and carbohydrates) in all tissues. Up-regulation of sugar biosynthesis in parallel to the increasing overproduction of reactive oxygen species (ROS) might enhance protein glycation, i.e. interaction of carbonyl compounds, reducing sugars and a-dicarbonyls with lysyl and arginyl side-chains yielding early (Amadori and Heyns compounds) and advanced glycation end-products (AGEs). Although the constitutive plant protein glycation patterns were characterized recently, the effects of environmental stress on AGE formation are unknown so far. To fill this gap, we present here a comprehensive in-depth study of the changes in Arabidopsis thaliana advanced glycated proteome related to osmotic stress. A 3 d application of osmotic stress revealed 31 stress-specifically and 12 differentially AGEmodified proteins, representing altogether 56 advanced glycation sites. Based on proteomic and metabolomic results, in combination with biochemical, enzymatic and gene expression analysis, we propose monosaccharide autoxidation as the main stress-related glycation mechanism, and glyoxal as the major glycation agent in plants subjected to drought.

AB - Among the environmental alterations accompanying oncoming climate changes, drought is the most important factor influencing crop plant productivity. In plants, water deficit ultimately results in the development of oxidative stress and accumulation of osmolytes (e.g. amino acids and carbohydrates) in all tissues. Up-regulation of sugar biosynthesis in parallel to the increasing overproduction of reactive oxygen species (ROS) might enhance protein glycation, i.e. interaction of carbonyl compounds, reducing sugars and a-dicarbonyls with lysyl and arginyl side-chains yielding early (Amadori and Heyns compounds) and advanced glycation end-products (AGEs). Although the constitutive plant protein glycation patterns were characterized recently, the effects of environmental stress on AGE formation are unknown so far. To fill this gap, we present here a comprehensive in-depth study of the changes in Arabidopsis thaliana advanced glycated proteome related to osmotic stress. A 3 d application of osmotic stress revealed 31 stress-specifically and 12 differentially AGEmodified proteins, representing altogether 56 advanced glycation sites. Based on proteomic and metabolomic results, in combination with biochemical, enzymatic and gene expression analysis, we propose monosaccharide autoxidation as the main stress-related glycation mechanism, and glyoxal as the major glycation agent in plants subjected to drought.

KW - Advanced glycation end-products (AGEs)

KW - Arabidopsis thaliana

KW - Crop quality

KW - Drought stress

KW - Food quality

KW - Glycation

KW - Label-free quantification

KW - Plant proteomics

KW - Two-dimensional chromatography

UR - http://www.scopus.com/inward/record.url?scp=85009967197&partnerID=8YFLogxK

U2 - 10.1093/jxb/erw395

DO - 10.1093/jxb/erw395

M3 - Article

C2 - 27856706

VL - 67

SP - 6283

EP - 6295

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 22

ER -