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Novel repressor of the human FMR1 gene - Identification of p56 human (GCC)n-binding protein as a Krüppel-like transcription factor ZF5. / Orlov, Sergey V.; Kuteykin-Teplyakov, Konstantin B.; Ignatovich, Irina A.; Dizhe, Ella B.; Mirgorodskaya, Olga A.; Grishin, Alexander V.; Guzhova, Olga B.; Prokhortchouk, Egor B.; Guliy, Pavel V.; Perevozchikov, Andrej P.

в: FEBS Journal, Том 274, № 18, 09.2007, стр. 4848-4862.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Orlov, SV, Kuteykin-Teplyakov, KB, Ignatovich, IA, Dizhe, EB, Mirgorodskaya, OA, Grishin, AV, Guzhova, OB, Prokhortchouk, EB, Guliy, PV & Perevozchikov, AP 2007, 'Novel repressor of the human FMR1 gene - Identification of p56 human (GCC)n-binding protein as a Krüppel-like transcription factor ZF5', FEBS Journal, Том. 274, № 18, стр. 4848-4862. https://doi.org/10.1111/j.1742-4658.2007.06006.x

APA

Orlov, S. V., Kuteykin-Teplyakov, K. B., Ignatovich, I. A., Dizhe, E. B., Mirgorodskaya, O. A., Grishin, A. V., Guzhova, O. B., Prokhortchouk, E. B., Guliy, P. V., & Perevozchikov, A. P. (2007). Novel repressor of the human FMR1 gene - Identification of p56 human (GCC)n-binding protein as a Krüppel-like transcription factor ZF5. FEBS Journal, 274(18), 4848-4862. https://doi.org/10.1111/j.1742-4658.2007.06006.x

Vancouver

Orlov SV, Kuteykin-Teplyakov KB, Ignatovich IA, Dizhe EB, Mirgorodskaya OA, Grishin AV и пр. Novel repressor of the human FMR1 gene - Identification of p56 human (GCC)n-binding protein as a Krüppel-like transcription factor ZF5. FEBS Journal. 2007 Сент.;274(18):4848-4862. https://doi.org/10.1111/j.1742-4658.2007.06006.x

Author

Orlov, Sergey V. ; Kuteykin-Teplyakov, Konstantin B. ; Ignatovich, Irina A. ; Dizhe, Ella B. ; Mirgorodskaya, Olga A. ; Grishin, Alexander V. ; Guzhova, Olga B. ; Prokhortchouk, Egor B. ; Guliy, Pavel V. ; Perevozchikov, Andrej P. / Novel repressor of the human FMR1 gene - Identification of p56 human (GCC)n-binding protein as a Krüppel-like transcription factor ZF5. в: FEBS Journal. 2007 ; Том 274, № 18. стр. 4848-4862.

BibTeX

@article{da3731b7dcf745f983893da5697472f3,
title = "Novel repressor of the human FMR1 gene - Identification of p56 human (GCC)n-binding protein as a Kr{\"u}ppel-like transcription factor ZF5",
abstract = "A series of relatively short (GCC)n triplet repeats (n = 3-30) located within regulatory regions of many mammalian genes may be considered as putative cis-acting transcriptional elements (GCC-elements). Fragile X-mental retardation syndrome is caused by an expansion of (GCC)n triplet repeats within the 5′-untranslated region of the human fragile X-mental retardation 1 (FMR1) gene. The present study aimed to characterize a novel human (GCC)n-binding protein and investigate its possible role in the regulation of the FMR1 gene. A novel human (GCC)n-binding protein, p56, was isolated and identified as a Kr{\"u}ppel-like transcription factor, ZF5, by MALDI-TOF analysis. The capacity of ZF5 to specifically interact with (GCC)n triplet repeats was confirmed by the electrophoretic mobility shift assay with purified recombinant ZF5 protein. In cotransfection experiments, ZF5 overexpression repressed activity of the GCC-element containing mouse ribosomal protein L32 gene promoter. Moreover, RNA interference assay results showed that endogenous ZF5 acts as a repressor of the human FMR1 gene. Thus, these data identify a new class of ZF5 targets, a subset of genes containing GCC-elements in their regulatory regions, and raise the question of whether transcription factor ZF5 is implicated in the pathogenesis of fragile X syndrome.",
keywords = "(GCC), FMR1, Fragile X syndrome, Triplet repeats, ZF5, Zinc finger transcription factors",
author = "Orlov, {Sergey V.} and Kuteykin-Teplyakov, {Konstantin B.} and Ignatovich, {Irina A.} and Dizhe, {Ella B.} and Mirgorodskaya, {Olga A.} and Grishin, {Alexander V.} and Guzhova, {Olga B.} and Prokhortchouk, {Egor B.} and Guliy, {Pavel V.} and Perevozchikov, {Andrej P.}",
year = "2007",
month = sep,
doi = "10.1111/j.1742-4658.2007.06006.x",
language = "English",
volume = "274",
pages = "4848--4862",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "18",

}

RIS

TY - JOUR

T1 - Novel repressor of the human FMR1 gene - Identification of p56 human (GCC)n-binding protein as a Krüppel-like transcription factor ZF5

AU - Orlov, Sergey V.

AU - Kuteykin-Teplyakov, Konstantin B.

AU - Ignatovich, Irina A.

AU - Dizhe, Ella B.

AU - Mirgorodskaya, Olga A.

AU - Grishin, Alexander V.

AU - Guzhova, Olga B.

AU - Prokhortchouk, Egor B.

AU - Guliy, Pavel V.

AU - Perevozchikov, Andrej P.

PY - 2007/9

Y1 - 2007/9

N2 - A series of relatively short (GCC)n triplet repeats (n = 3-30) located within regulatory regions of many mammalian genes may be considered as putative cis-acting transcriptional elements (GCC-elements). Fragile X-mental retardation syndrome is caused by an expansion of (GCC)n triplet repeats within the 5′-untranslated region of the human fragile X-mental retardation 1 (FMR1) gene. The present study aimed to characterize a novel human (GCC)n-binding protein and investigate its possible role in the regulation of the FMR1 gene. A novel human (GCC)n-binding protein, p56, was isolated and identified as a Krüppel-like transcription factor, ZF5, by MALDI-TOF analysis. The capacity of ZF5 to specifically interact with (GCC)n triplet repeats was confirmed by the electrophoretic mobility shift assay with purified recombinant ZF5 protein. In cotransfection experiments, ZF5 overexpression repressed activity of the GCC-element containing mouse ribosomal protein L32 gene promoter. Moreover, RNA interference assay results showed that endogenous ZF5 acts as a repressor of the human FMR1 gene. Thus, these data identify a new class of ZF5 targets, a subset of genes containing GCC-elements in their regulatory regions, and raise the question of whether transcription factor ZF5 is implicated in the pathogenesis of fragile X syndrome.

AB - A series of relatively short (GCC)n triplet repeats (n = 3-30) located within regulatory regions of many mammalian genes may be considered as putative cis-acting transcriptional elements (GCC-elements). Fragile X-mental retardation syndrome is caused by an expansion of (GCC)n triplet repeats within the 5′-untranslated region of the human fragile X-mental retardation 1 (FMR1) gene. The present study aimed to characterize a novel human (GCC)n-binding protein and investigate its possible role in the regulation of the FMR1 gene. A novel human (GCC)n-binding protein, p56, was isolated and identified as a Krüppel-like transcription factor, ZF5, by MALDI-TOF analysis. The capacity of ZF5 to specifically interact with (GCC)n triplet repeats was confirmed by the electrophoretic mobility shift assay with purified recombinant ZF5 protein. In cotransfection experiments, ZF5 overexpression repressed activity of the GCC-element containing mouse ribosomal protein L32 gene promoter. Moreover, RNA interference assay results showed that endogenous ZF5 acts as a repressor of the human FMR1 gene. Thus, these data identify a new class of ZF5 targets, a subset of genes containing GCC-elements in their regulatory regions, and raise the question of whether transcription factor ZF5 is implicated in the pathogenesis of fragile X syndrome.

KW - (GCC)

KW - FMR1

KW - Fragile X syndrome

KW - Triplet repeats

KW - ZF5

KW - Zinc finger transcription factors

UR - http://www.scopus.com/inward/record.url?scp=34548402660&partnerID=8YFLogxK

U2 - 10.1111/j.1742-4658.2007.06006.x

DO - 10.1111/j.1742-4658.2007.06006.x

M3 - Article

C2 - 17714511

AN - SCOPUS:34548402660

VL - 274

SP - 4848

EP - 4862

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 18

ER -

ID: 91966383