Standard

Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula. / Potsenkovskaia, Elina; Tvorogova, Varvara; Yakovleva, Daria; Zlydneva, Natalia; Lutova, Ludmila.

в: Plant Gene, Том 31, 100364, 09.2022.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Potsenkovskaia, E, Tvorogova, V, Yakovleva, D, Zlydneva, N & Lutova, L 2022, 'Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula', Plant Gene, Том. 31, 100364. https://doi.org/10.1016/j.plgene.2022.100364

APA

Potsenkovskaia, E., Tvorogova, V., Yakovleva, D., Zlydneva, N., & Lutova, L. (2022). Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula. Plant Gene, 31, [100364]. https://doi.org/10.1016/j.plgene.2022.100364

Vancouver

Potsenkovskaia E, Tvorogova V, Yakovleva D, Zlydneva N, Lutova L. Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula. Plant Gene. 2022 Сент.;31. 100364. https://doi.org/10.1016/j.plgene.2022.100364

Author

Potsenkovskaia, Elina ; Tvorogova, Varvara ; Yakovleva, Daria ; Zlydneva, Natalia ; Lutova, Ludmila. / Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula. в: Plant Gene. 2022 ; Том 31.

BibTeX

@article{1fc86488002e492fb16f1e67844a3141,
title = "Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula",
abstract = "Somatic embryogenesis is widely used in biotechnology as a regeneration process conditioning many protocols for plant transformation and in vitro propagation. The main objective of this study was to search for new potential somatic embryogenesis regulators among the genes belonging to the NF-Y family and to analyse the mechanism of their action in Medicago truncatula. We found that two LEC1-type NF-YB genes, MtNF-YB3 and 10, are highly expressed in the calli of the embryogenic 2HA line during somatic embryogenesis in M. truncatula. Overexpression of these genes did not have a significant effect on the somatic embryogenesis capacity. Several genes from NF-YA and C groups with high expression levels during somatic embryogenesis were found. We demonstrated that transcription factors, encoded by some of them, were able to interact with MtNF-YB3 and 10, and overexpression of one of these genes, MtNF-YA3, increased callus weight. The results obtained provide new information about NF-Y genes functioning during plant regeneration and can be used for the search of new somatic embryogenesis stimulants.",
keywords = "LEC1, NF-Y complex, Protein-protein interactions, Somatic embryogenesis capacity, Yeast two-hybrid system",
author = "Elina Potsenkovskaia and Varvara Tvorogova and Daria Yakovleva and Natalia Zlydneva and Ludmila Lutova",
note = "Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",
year = "2022",
month = sep,
doi = "10.1016/j.plgene.2022.100364",
language = "English",
volume = "31",
journal = "Plant Gene",
issn = "2352-4073",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula

AU - Potsenkovskaia, Elina

AU - Tvorogova, Varvara

AU - Yakovleva, Daria

AU - Zlydneva, Natalia

AU - Lutova, Ludmila

N1 - Publisher Copyright: © 2022 Elsevier B.V.

PY - 2022/9

Y1 - 2022/9

N2 - Somatic embryogenesis is widely used in biotechnology as a regeneration process conditioning many protocols for plant transformation and in vitro propagation. The main objective of this study was to search for new potential somatic embryogenesis regulators among the genes belonging to the NF-Y family and to analyse the mechanism of their action in Medicago truncatula. We found that two LEC1-type NF-YB genes, MtNF-YB3 and 10, are highly expressed in the calli of the embryogenic 2HA line during somatic embryogenesis in M. truncatula. Overexpression of these genes did not have a significant effect on the somatic embryogenesis capacity. Several genes from NF-YA and C groups with high expression levels during somatic embryogenesis were found. We demonstrated that transcription factors, encoded by some of them, were able to interact with MtNF-YB3 and 10, and overexpression of one of these genes, MtNF-YA3, increased callus weight. The results obtained provide new information about NF-Y genes functioning during plant regeneration and can be used for the search of new somatic embryogenesis stimulants.

AB - Somatic embryogenesis is widely used in biotechnology as a regeneration process conditioning many protocols for plant transformation and in vitro propagation. The main objective of this study was to search for new potential somatic embryogenesis regulators among the genes belonging to the NF-Y family and to analyse the mechanism of their action in Medicago truncatula. We found that two LEC1-type NF-YB genes, MtNF-YB3 and 10, are highly expressed in the calli of the embryogenic 2HA line during somatic embryogenesis in M. truncatula. Overexpression of these genes did not have a significant effect on the somatic embryogenesis capacity. Several genes from NF-YA and C groups with high expression levels during somatic embryogenesis were found. We demonstrated that transcription factors, encoded by some of them, were able to interact with MtNF-YB3 and 10, and overexpression of one of these genes, MtNF-YA3, increased callus weight. The results obtained provide new information about NF-Y genes functioning during plant regeneration and can be used for the search of new somatic embryogenesis stimulants.

KW - LEC1

KW - NF-Y complex

KW - Protein-protein interactions

KW - Somatic embryogenesis capacity

KW - Yeast two-hybrid system

UR - http://www.scopus.com/inward/record.url?scp=85131432763&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/5b7cb636-d332-3ed9-a9b2-ae8210fac476/

U2 - 10.1016/j.plgene.2022.100364

DO - 10.1016/j.plgene.2022.100364

M3 - Article

AN - SCOPUS:85131432763

VL - 31

JO - Plant Gene

JF - Plant Gene

SN - 2352-4073

M1 - 100364

ER -

ID: 100072218