Еvaluation of histamine levels in saliva can be used in characterization of Helicobacter pylori extragastric manifestations. Therefore, a sensitive, simple, and low-cost method for determining histamine concentrations is needed for point-of-care diagnostics. In this work, we studied the use of glassy carbon disk electrode, bulk graphite composite electrode and graphite screen-printed electrode modified with CuO nanoparticles and CuSO4 towards histamine quantification by cyclic voltammetry. The best results were obtained using the screen-printed sensor, whose working electrode had been modified by CuSO4 aqueous solution and covered with Nafion-like polymer film. The developed sensor showed a 5·10-9 M detection limit with a broad linear range from 5·10-9 M till 1·10-6 M of histamine in PBS, which is enough for reliable saliva analysis after the simple sample preparation. The analysis of real saliva samples revealed no significant interfering matrix effect. The time of analysis, including sample preparation, doesn’t exceed 15 minutes.