The conditions for synthesis of conductive copper and nickel microstructures using laser-induced deposition technique were determined and electrocatalytic activity of these materials towards D-glucose was investigated. It was found out that formation of these conductive electrodes upon laser irradiation occurs only in solutions containing OH- coordinating ligands. Coordination via other functional groups (including carboxyl and amino) does not lead to synthesis of conductive structures. Topology and composition of copper and nickel deposits were observed using scanning electron microscopy and EDX analysis, respectively. The electrochemical properties of the synthesized structures were studied using cyclic voltamperometry and amperometry. The studied materials display linear dependencies of the Faraday current vs. concentration between 2 μM and 1.1 mM for nickel and 0.25 mM and 3 mM for copper.