TY - JOUR

T1 - NK-92 cells change their phenotype and function when cocultured with IL-15, IL-18 and trophoblast cells

AU - Mikhailova, Valentina

AU - Khokhlova, Evgeniia

AU - Grebenkina, Polina

AU - Salloum, Zeina

AU - Nikolaenkov, Igor

AU - Markova, Kseniya

AU - Davidova, Alina

AU - Selkov, Sergey

AU - Sokolov, Dmitriy

N1 - Publisher Copyright: © 2021 Elsevier GmbH

PY - 2021/9/1

Y1 - 2021/9/1

N2 - NK cell development is affected by their cellular microenvironment and cytokines, including IL-15 and IL-18. NK cells can differentiate in secondary lymphoid organs, liver and within the uterus in close contact with trophoblast cells. The aim was to evaluate changes in the NK cell phenotype and function in the presence of IL-15, IL-18 and JEG-3, a trophoblast cell line. When cocultured with JEG-3 cells, IL-15 caused an increase in the number of NKG2D+ NK-92 cells and the intensity of CD127 expression. IL-18 stimulates an increase in the amount of NKp44+ NK-92 cells and in the intensity of NKp44 expression by pNK in the presence of trophoblast cells. NK-92 cell cytotoxic activity against JEG-3 cells increased only in presence of IL-18. Data on changes in the cytotoxic activity of NK-92 cells against JEG-3 cells in the presence of IL-15 and IL-18 indicate the modulation of NK cell function both by the cytokine microenvironment and directly by target cells. IL-15 and IL-18 were present in conditioned media (CM) from 1st and 3rd trimester placentas. In the presence of 1st trimester CM and JEG-3 cells, NK-92 cells showed an increase in the intensity of NKG2D expression. In the presence of 3rd trimester CM and JEG-3 cells, a decrease in the expression of NKG2D by NK-92 cells was observed. Thus, culturing of NK-92 cells with JEG-3 trophoblast cells stimulated a pronounced change in the NK cell phenotype, bringing it closer to the decidual NK cell-like phenotype.

AB - NK cell development is affected by their cellular microenvironment and cytokines, including IL-15 and IL-18. NK cells can differentiate in secondary lymphoid organs, liver and within the uterus in close contact with trophoblast cells. The aim was to evaluate changes in the NK cell phenotype and function in the presence of IL-15, IL-18 and JEG-3, a trophoblast cell line. When cocultured with JEG-3 cells, IL-15 caused an increase in the number of NKG2D+ NK-92 cells and the intensity of CD127 expression. IL-18 stimulates an increase in the amount of NKp44+ NK-92 cells and in the intensity of NKp44 expression by pNK in the presence of trophoblast cells. NK-92 cell cytotoxic activity against JEG-3 cells increased only in presence of IL-18. Data on changes in the cytotoxic activity of NK-92 cells against JEG-3 cells in the presence of IL-15 and IL-18 indicate the modulation of NK cell function both by the cytokine microenvironment and directly by target cells. IL-15 and IL-18 were present in conditioned media (CM) from 1st and 3rd trimester placentas. In the presence of 1st trimester CM and JEG-3 cells, NK-92 cells showed an increase in the intensity of NKG2D expression. In the presence of 3rd trimester CM and JEG-3 cells, a decrease in the expression of NKG2D by NK-92 cells was observed. Thus, culturing of NK-92 cells with JEG-3 trophoblast cells stimulated a pronounced change in the NK cell phenotype, bringing it closer to the decidual NK cell-like phenotype.

KW - JEG-3

KW - K562

KW - NK cells

KW - NKG2D

KW - NKp44

KW - Trophoblast

KW - NATURAL-KILLER-CELLS

KW - MEDIATED CYTOTOXICITY

KW - DECIDUAL NK CELLS

KW - IDENTIFICATION

KW - HLA-G

KW - MATERNAL-FETAL INTERFACE

KW - INTERLEUKIN-15

KW - ACTIVATING RECEPTORS

KW - EXPRESSION

KW - LINE NK-92

UR - http://www.scopus.com/inward/record.url?scp=85112006173&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/2257619f-c5a0-31fd-82fe-ed09cdc3e842/

U2 - 10.1016/j.imbio.2021.152125

DO - 10.1016/j.imbio.2021.152125

M3 - Article

AN - SCOPUS:85112006173

VL - 226

JO - Immunobiology

JF - Immunobiology

SN - 0171-2985

IS - 5

M1 - 152125

ER -