TY - JOUR

T1 - Newly identified diversity of Dinomycetaceae (Rhizophydiales, Chytridiomycota), a family of fungal parasites of marine dinoflagellates

AU - Fernández-Valero, A.D.

AU - Karpov, S.A.

AU - Sampedro, N.

AU - Gordi, J.

AU - Timoneda, N.

AU - Garcés, E.

AU - Reñé, A.

N1 - Export Date: 21 March 2024 CODEN: EJPRE Адрес для корреспонденции: Fernández-Valero, A.D.; Departament de Biologia Marina i Oceanografia, Pg. Marítim de la Barceloneta, 37-49, Catalonia, Spain; эл. почта: alanfdez.valero@gmail.com Сведения о финансировании: Ministerio de Ciencia, Innovación y Universidades, MCIU, PRE2018-084893 Сведения о финансировании: Saint Petersburg State University, SPbU, 115624290, 122031100260-0 Сведения о финансировании: Ministerio de Ciencia e Innovación, MICINN, CEX2019-000928-S, SMART PID2020-112978GB-I00 Сведения о финансировании: Universitat de Barcelona, UB Сведения о финансировании: Russian Science Foundation, RSF, 21–74–20089 Сведения о финансировании: Norsk institutt for naturforskning, NINA Текст о финансировании 1: This study was funded by the Spanish MICINN Project SMART PID2020-112978GB-I00, with institutional support from the “Severo Ochoa Centre of Excellence” (CEX2019-000928-S). A. D. Fernández-Valero was funded by the MICIU grant “Ayudas para contratos predoctorales para la formación de doctores 2018” (PRE2018-084893). This work was partly supported by the Russian Science Foundation, grant no. 21–74–20089 (light and electron microscopy analysis, manuscript writing). SAK thanks Saint-Petersburg State University for a research grant (project ID Pure 115624290) and ZIN RAS program 122031100260-0. We thank the TEM-SEM Electron Microscopy Unit from Scientific and Technological Centers (CCiTUB), Universitat de Barcelona, and its staff for their support and advice during the TEM studies. We thank Dr. J-M. Rintala (University of Helsinki, Finland) and Dr. M. Majaneva (Norwegian Institute for Nature Research, Norway) for providing metadata from their metabarcoding dataset. We also thank Dr. M. Antó and Dr. I. Ruiz (Institut de Biologia Evolutiva IBE-CSIC) for their time and for providing access to their laboratory equipment. Текст о финансировании 2: This study was funded by the Spanish MICINN Project SMART PID2020-112978GB-I00, with institutional support from the “Severo Ochoa Centre of Excellence” (CEX2019-000928-S). A. D. Fernández-Valero was funded by the MICIU grant “Ayudas para contratos predoctorales para la formación de doctores 2018” (PRE2018-084893). This work was partly supported by the Russian Science Foundation, grant no. 21–74–20089 (light and electron microscopy analysis, manuscript writing). SAK thanks Saint-Petersburg State University for a research grant (project ID Pure 115624290) and ZIN RAS program 122031100260-0. We thank the TEM-SEM Electron Microscopy Unit from Scientific and Technological Centers (CCiTUB), Universitat de Barcelona, and its staff for their support and advice during the TEM studies. We thank Dr. J-M. Rintala (University of Helsinki, Finland) and Dr. M. Majaneva (Norwegian Institute for Nature Research, Norway) for providing metadata from their metabarcoding dataset. We also thank Dr. M. Antó and Dr. I. Ruiz (Institut de Biologia Evolutiva IBE-CSIC) for their time and for providing access to their laboratory equipment. Molecular sequences generated in this study are publicly available in GenBank. Phylogenetic alignments analysed in the present study are available from the corresponding author upon request.

PY - 2024/4/1

Y1 - 2024/4/1

N2 - We identified two new parasite species of Chytridiomycota isolated during blooms of the dinoflagellate Alexandrium minutum in the coastal Mediterranean Sea. Light and electron microscopy together with molecular characterization of the nuclear 18S, ITS, and 28S rDNA regions led to their identification as two new species, Dinomyces gilberthii and Paradinomyces evelyniae, both belonging to the family Dinomycetaceae, order Rhizophydiales. Dinomyces gilberthii differs from the previously described D. arenysensis by the presence of discharge papillae and the development of a drop-shaped sporangium. Paradinomyces evelyniae differs from the previously described P. triforaminorum by the prominent lipid globule present in early sporangia and by the pointed end producing a rhizoid. The two chytrids differed in their geographical distribution. Dinomyces gilberthii was detected in several Mediterranean habitats, including harbours and beaches, and was particularly prevalent during summer dinoflagellate blooms. Its widespread occurrence in coastal ecosystems suggested a high level of adaptability to this environment. Paradinomyces evelyniae had a more restricted distribution in the coastal-marine environment, occurring in harbour sediments and only occasionally in the water column during winter and early spring. Paradinomyces evelyniae has previously been detected in the Baltic Sea, suggesting that its distribution encompasses contrasting coastal environments, although its presence is rare. © 2024 The Author(s)

AB - We identified two new parasite species of Chytridiomycota isolated during blooms of the dinoflagellate Alexandrium minutum in the coastal Mediterranean Sea. Light and electron microscopy together with molecular characterization of the nuclear 18S, ITS, and 28S rDNA regions led to their identification as two new species, Dinomyces gilberthii and Paradinomyces evelyniae, both belonging to the family Dinomycetaceae, order Rhizophydiales. Dinomyces gilberthii differs from the previously described D. arenysensis by the presence of discharge papillae and the development of a drop-shaped sporangium. Paradinomyces evelyniae differs from the previously described P. triforaminorum by the prominent lipid globule present in early sporangia and by the pointed end producing a rhizoid. The two chytrids differed in their geographical distribution. Dinomyces gilberthii was detected in several Mediterranean habitats, including harbours and beaches, and was particularly prevalent during summer dinoflagellate blooms. Its widespread occurrence in coastal ecosystems suggested a high level of adaptability to this environment. Paradinomyces evelyniae had a more restricted distribution in the coastal-marine environment, occurring in harbour sediments and only occasionally in the water column during winter and early spring. Paradinomyces evelyniae has previously been detected in the Baltic Sea, suggesting that its distribution encompasses contrasting coastal environments, although its presence is rare. © 2024 The Author(s)

KW - Chytridiomycota

KW - Coastal ecosystems

KW - Dinoflagellate

KW - Fungal parasites

KW - Fungi

KW - Molecular phylogeny

KW - Ultrastructure

UR - https://www.mendeley.com/catalogue/fc71934f-1853-3215-963f-8f0a214ba706/

U2 - 10.1016/j.ejop.2024.126053

DO - 10.1016/j.ejop.2024.126053

M3 - статья

VL - 93

JO - European Journal of Protistology

JF - European Journal of Protistology

SN - 0932-4739

M1 - 126053

ER -