TY - JOUR

T1 - New Member of Gromochytriales (Chytridiomycetes)—Apiochytrium granulosporum nov. gen. et sp.

AU - Karpov, Sergey A.

AU - Moreira, David

AU - Mamkaeva, Maria A.

AU - Popova, Olga V.

AU - Aleoshin, Vladimir V.

AU - López-García, Purificación

PY - 2019/7

Y1 - 2019/7

N2 - Molecular phylogenetic analysis of 18S rRNA gene sequences of nearly any species of Chytridiomycota has typically challenged traditional classification and triggered taxonomic revision. This has often led to the establishment of new taxa which, normally, appears well supported by zoospore ultrastructure, which provides diagnostic characters. To construct a meaningful and comprehensive classification of Chytridiomycota, the combination of molecular phylogenies and morphological studies of traditionally defined chytrid species is needed. In this work, we have studied morphological and ultrastructural features based on light and transmission electron microscopy as well as molecular phylogenetic analysis of a parasite (strain X-124 CCPP ZIN RAS) morphologically similar to Rhizophydium granulosporum living on the yellow-green alga Tribonema gayanum. Phylogenetic analysis of the 18S rRNA gene sequence of this strain supports that it represents a new genus and species affiliated to the recently established order Gromochytriales. The ultrastructure of X-124 confirms its phylogenetic position sister to Gromochytrium and serves as the basis for the description of the new genus and species Apiochytrium granulosporum. The 18S rRNA gene of A. granulosporum contains a S943 group I intron that carries a homing endonuclease pseudogene.

AB - Molecular phylogenetic analysis of 18S rRNA gene sequences of nearly any species of Chytridiomycota has typically challenged traditional classification and triggered taxonomic revision. This has often led to the establishment of new taxa which, normally, appears well supported by zoospore ultrastructure, which provides diagnostic characters. To construct a meaningful and comprehensive classification of Chytridiomycota, the combination of molecular phylogenies and morphological studies of traditionally defined chytrid species is needed. In this work, we have studied morphological and ultrastructural features based on light and transmission electron microscopy as well as molecular phylogenetic analysis of a parasite (strain X-124 CCPP ZIN RAS) morphologically similar to Rhizophydium granulosporum living on the yellow-green alga Tribonema gayanum. Phylogenetic analysis of the 18S rRNA gene sequence of this strain supports that it represents a new genus and species affiliated to the recently established order Gromochytriales. The ultrastructure of X-124 confirms its phylogenetic position sister to Gromochytrium and serves as the basis for the description of the new genus and species Apiochytrium granulosporum. The 18S rRNA gene of A. granulosporum contains a S943 group I intron that carries a homing endonuclease pseudogene.

KW - Chytrids

KW - endonuclease pseudogene

KW - intron

KW - molecular phylogeny

KW - ultrastructure

KW - ORDER

KW - EVOLUTION

KW - CHYTRIDIOMYCOTA

KW - 18S

KW - MULTIPLE SEQUENCE ALIGNMENT

KW - MOLECULAR PHYLOGENY

KW - TOOL

UR - http://www.scopus.com/inward/record.url?scp=85058179245&partnerID=8YFLogxK

UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/jeu.12702?af=R

UR - http://www.mendeley.com/research/new-member-gromochytriales-chytridiomycetes-x2013-apiochytrium-granulosporum-nov-gen-et-sp

U2 - 10.1111/jeu.12702

DO - 10.1111/jeu.12702

M3 - Article

C2 - 30460733

AN - SCOPUS:85058179245

VL - 66

SP - 582

EP - 591

JO - Journal of Eukaryotic Microbiology

JF - Journal of Eukaryotic Microbiology

SN - 1066-5234

IS - 4

ER -