TY - JOUR

T1 - New Fluorescent Dye for the Detection of Zn2+ in Living Cells and Fixed Sections of the Rat Pancreas

AU - Krinochkin, Alexey

AU - Valieva, Maria

AU - Starnovskaya, Ekaterina

AU - Slovesnova, Nataliya

AU - Minin, Artem

AU - Belousova, Anna

AU - Sadieva, Leila

AU - Taniya, Olga

AU - Khasanov, Albert

AU - Novikov, Alexander

AU - Bruskov, Vitaly

AU - Vatolina, Svetlana

AU - Kopchuk, Dmitry

AU - Slepukhin, Pavel

AU - Sharutin, Vladimir

AU - Zyryanov, Grigory

PY - 2024/2/13

Y1 - 2024/2/13

N2 - We report the synthesis and characterization of a new 4-methoxyphenyl-2,2’-bipyridine-based ligand, such as 12, bearing dipicolylaminomethyl core as a receptor unit, as a probe for the fluorescence “turn-on” detection of Zn2+. Thus, in the presence of Zn2+ the probe 12 exhibited a fluorescence enhancement with a Stokes shift of ~ 180 nm and photoluminescence quantum yields value of ~ 1.0. In addition, 12 exhibited higher binding constant for Zn2+ (~ 2 × 105 M−1) with the LOD reaching the nanomolar level (~ 0.1 × 10–9 M) compare to the previously reported probe 1. The stoichiometry and structure of the [Zn(12)]2+ and [Zn(1)]2+ complexes were supported by XRD analysis, DFT calculations and 1H NMR experiments. It was postulated that, as a result of binding of Zn2+, the sample exhibited a bright “on” state via the PET-ICT processes. Molecular docking studies and confocal fluorescence microscopy experiments demonstrated that the probe 12 could be used for the fluorescence detection of Zn2+ not only in artificially enriched with zinc salts live cells, but also in fixed tissues with cations are in a bound state. The high binding constant of compound 12 to Zn2+ cation allows it to be used for the accurate localization of pancreatic beta cells (islets of Langerhans). Graphical Abstract: (Figure presented.).

AB - We report the synthesis and characterization of a new 4-methoxyphenyl-2,2’-bipyridine-based ligand, such as 12, bearing dipicolylaminomethyl core as a receptor unit, as a probe for the fluorescence “turn-on” detection of Zn2+. Thus, in the presence of Zn2+ the probe 12 exhibited a fluorescence enhancement with a Stokes shift of ~ 180 nm and photoluminescence quantum yields value of ~ 1.0. In addition, 12 exhibited higher binding constant for Zn2+ (~ 2 × 105 M−1) with the LOD reaching the nanomolar level (~ 0.1 × 10–9 M) compare to the previously reported probe 1. The stoichiometry and structure of the [Zn(12)]2+ and [Zn(1)]2+ complexes were supported by XRD analysis, DFT calculations and 1H NMR experiments. It was postulated that, as a result of binding of Zn2+, the sample exhibited a bright “on” state via the PET-ICT processes. Molecular docking studies and confocal fluorescence microscopy experiments demonstrated that the probe 12 could be used for the fluorescence detection of Zn2+ not only in artificially enriched with zinc salts live cells, but also in fixed tissues with cations are in a bound state. The high binding constant of compound 12 to Zn2+ cation allows it to be used for the accurate localization of pancreatic beta cells (islets of Langerhans). Graphical Abstract: (Figure presented.).

KW - 2,2’-Bipyridine

KW - Confocal microscopy

KW - Insulin hexamer

KW - Rat pancreas

KW - Sensor

KW - Zn2+ detection

UR - https://www.mendeley.com/catalogue/1174b4e8-1a4d-31ea-bb74-d8c1b5bafb08/

U2 - 10.1007/s10895-024-03603-1

DO - 10.1007/s10895-024-03603-1

M3 - Article

JO - Journal of Fluorescence

JF - Journal of Fluorescence

SN - 1053-0509

ER -