TY - JOUR

T1 - Multi-site binding of human nuclear protein to the Alu-family repeated DNA

AU - Podgornaya, O.I.

AU - Perelygina, L.M.

AU - Tomilin, N.V.

PY - 1988

Y1 - 1988

N2 - Nuclear protein which selectively binds to the Alu-family DNA repeat (AFR, Blur8) is partially purified from human HeLa cells using a gel retention assay. At low protein concentrations only a single complex of the protein with AFR is formed (CII). Increasing protein concentrations lead to the gradual disappearance of CII, being replaced by complexes with higher (CI) and lower (CIII, CIV) electrophoretic mobilities. Differential binding of AFR restriction subfragments indicates that multiple protein-binding sites are present within AFR. We discuss two models explaining the anomalous electrophoretic mobility of CII by DNA bending or looping upon cooperative multi-site binding of the protein to AFR.

AB - Nuclear protein which selectively binds to the Alu-family DNA repeat (AFR, Blur8) is partially purified from human HeLa cells using a gel retention assay. At low protein concentrations only a single complex of the protein with AFR is formed (CII). Increasing protein concentrations lead to the gradual disappearance of CII, being replaced by complexes with higher (CI) and lower (CIII, CIV) electrophoretic mobilities. Differential binding of AFR restriction subfragments indicates that multiple protein-binding sites are present within AFR. We discuss two models explaining the anomalous electrophoretic mobility of CII by DNA bending or looping upon cooperative multi-site binding of the protein to AFR.

KW - DNA-binding protein

KW - Alu-family DNA repeat

U2 - http://dx.doi.org/10.1016/0014-5793(88)80394-6

DO - http://dx.doi.org/10.1016/0014-5793(88)80394-6

M3 - Article

VL - 232

SP - 99

EP - 102

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -