Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Multiple Euryhaline Lineages of Centrohelids (Haptista: Centroplasthelida) in Inland Saline Waters Revealed with Metabarcoding. / Gerasimova, Elena A.; Plotnikov, Andrey O. ; Khlopko, Yuriy A. ; Zlatogursky, Vasily V. .
в: Journal of Eukaryotic Microbiology, Том 67, № 2, 01.03.2020, стр. 223 - 231.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Multiple Euryhaline Lineages of Centrohelids (Haptista: Centroplasthelida) in Inland Saline Waters Revealed with Metabarcoding
AU - Gerasimova, Elena A.
AU - Plotnikov, Andrey O.
AU - Khlopko, Yuriy A.
AU - Zlatogursky, Vasily V.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - The diversity of centrohelids in inland saline waters was studied with metabarcoding for the first time. The fragment of V6–V7 regions of 18S rDNA was sequenced with newly designed primers. Obtained OTUs were identified with molecular phylogenetic analysis and comparison of the signatures in 39es9 hairpin of V7. The obtained data included some OTUs, which could be attributed to four described species, but the majority belonged to previously established or novel environmental clades. Along with some presumably marine/brackish clades and freshwater/low salinity (0–2 ppt) clades, seven presumable species demonstrating broad (from 1–2 up to 78 ppt) salinity tolerance were detected. A number of OTUs belonged to Raphidocystis contractilis, which is known from three independent findings in brackish habitats only. Thus, it was assumed that this species is stenohaline and specifically adapted to salinity 5–15 ppt. The high level of salinity tolerance was suggested for centrohelids before based on morphology, which was used to justify their cosmopolitan distribution. Later these views were criticized based on environmental sequencing, but the results of the current survey indicate, that at least some species are present at salinities from almost freshwater (1–2 ppt) to twice oceanic (78 ppt) and are presumably capable of overcoming oceanic salinity barriers for their distribution.
AB - The diversity of centrohelids in inland saline waters was studied with metabarcoding for the first time. The fragment of V6–V7 regions of 18S rDNA was sequenced with newly designed primers. Obtained OTUs were identified with molecular phylogenetic analysis and comparison of the signatures in 39es9 hairpin of V7. The obtained data included some OTUs, which could be attributed to four described species, but the majority belonged to previously established or novel environmental clades. Along with some presumably marine/brackish clades and freshwater/low salinity (0–2 ppt) clades, seven presumable species demonstrating broad (from 1–2 up to 78 ppt) salinity tolerance were detected. A number of OTUs belonged to Raphidocystis contractilis, which is known from three independent findings in brackish habitats only. Thus, it was assumed that this species is stenohaline and specifically adapted to salinity 5–15 ppt. The high level of salinity tolerance was suggested for centrohelids before based on morphology, which was used to justify their cosmopolitan distribution. Later these views were criticized based on environmental sequencing, but the results of the current survey indicate, that at least some species are present at salinities from almost freshwater (1–2 ppt) to twice oceanic (78 ppt) and are presumably capable of overcoming oceanic salinity barriers for their distribution.
KW - molecular phylogeny
KW - high‐throughput sequencing
KW - DNA‐metabarcoding
KW - heliozoa
KW - halophilic protists
KW - 18S rDNA
KW - DNA metabarcoding
KW - high-throughput sequencing
KW - N.
KW - PHYLOGENY
KW - HELIOZOA
KW - AXOPODIAL CONTRACTION
KW - EVOLUTION
KW - RAPHIDIOPHRYS-HETEROPHRYOIDEA
KW - DIVERSITY
KW - SP-NOV
UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/jeu.12776
UR - http://www.scopus.com/inward/record.url?scp=85076719131&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/multiple-euryhaline-lineages-centrohelids-haptista-centroplasthelida-inland-saline-waters-revealed-m
U2 - 10.1111/jeu.12776
DO - 10.1111/jeu.12776
M3 - Article
VL - 67
SP - 223
EP - 231
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
SN - 1066-5234
IS - 2
ER -
ID: 48980666