TY - JOUR

T1 - Morphology and Molecular Phylogeny of Coelomic Gregarines (Apicomplexa) with Different Types of Motility

T2 - Urospora ovalis and U. travisiae from the Polychaete Travisia forbesii

AU - Diakin, Andrei

AU - Paskerova, Gita G.

AU - Simdyanov, Timur G.

AU - Aleoshin, Vladimir V.

AU - Valigurová, Andrea

N1 - Funding Information: The authors are grateful to the staff of the Marine Biological Station of Saint-Petersburg State University (MBS SPbSU) and the Nikolai Pertsov White Sea Biological Station of Lomonosov Moscow State University (WSBS MSU) for providing them with on-site facilities for field sampling and material processing. The authors are greatly indebted to members of the Laboratory of Electron Microscopy (Institute of Parasitology, BC ASCR in České Budějovice) for their technical assistance. AD and GGP would like to thank Andrej Dobrovolskij (Dept. of Invertebrate Zoology, St. Petersburg State University) for initiating this study, and also Vladimir Semenov and Tatiana Kharkievitch (Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences) for their assistance with electron microscopy. A part of the present study was performed at the Resource Centres (Culture Collections of Microorganisms, Molecular and Cell Technologies, Observatory of Environmental Safety) of St. Petersburg State University. GGP is grateful to Professor Rudolf Entzeroth for providing facilities for her research at Dresden Technical University (TUD) and to Markus Gunther (TUD) for his assistance with SEM. DNA sequencing was performed at the “Genome” DNA sequencing centre (Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, www.genome-centre.ru). AV and AD were funded by project No. GBP505/12/G112 from the Czech Science Foundation (ECIP - Centre of Excellence) and acknowledge support from the Department of Botany and Zoology, Faculty of Science, Masaryk University towards the preparation of this manuscript. GGP was supported by St. Petersburg State University grants (1.42.1493.2015, 1.42.1099.2016), a Michail Lomonosov stipendium (DAAD), and TUD grants. TGS was supported by grants from the Council of the President of the Russian Federation (NSh-7770.2016.4) and from the Russian Foundation of Basic Research (15-29-02601). The phylogenetic analysis in this study was supported by Russian Scientific Foundation grant No. 14-50-00029. The authors also grateful to Matthew Nicholls for English proof-reading. Publisher Copyright: © 2016 Elsevier GmbH. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Urosporids (Apicomplexa: Urosporidae) are eugregarines that parasitise marine invertebrates, such as annelids, molluscs, nemerteans and echinoderms, inhabiting their coelom and intestine. Urosporids exhibit considerable morphological plasticity, which correlates with their different modes of motility and variations in structure of their cortical zone, according to the localisation within the host. The gregarines Urospora ovalis and U. travisiae from the marine polychaete Travisia forbesii were investigated with an emphasis on their general morphology and phylogenetic position. Solitary ovoid trophozoites and syzygies of U. ovalis were located free in the host coelom and showed metabolic activity, a non-progressive movement with periodic changes of the cell shape. Solitary trophozoites of U. travisiae, attached to the host tissue or free floating in the coelom, were V-shaped. Detached trophozoites demonstrated gliding motility, a progressive movement without observable cell body changes. In both gregarines, the cortex formed numerous epicytic folds, but superfolds appeared exclusively on the surface of U. ovalis during metabolic activity. SSU rDNA sequences obtained from U. ovalis and U. travisiae revealed that they belong to the Lecudinoidea clade; however, they are not affiliated with other coelomic urosporids (Pterospora spp. and Lithocystis spp.), but surprisingly with intestinal lecudinids (Difficilina spp.) parasitising nemerteans.

AB - Urosporids (Apicomplexa: Urosporidae) are eugregarines that parasitise marine invertebrates, such as annelids, molluscs, nemerteans and echinoderms, inhabiting their coelom and intestine. Urosporids exhibit considerable morphological plasticity, which correlates with their different modes of motility and variations in structure of their cortical zone, according to the localisation within the host. The gregarines Urospora ovalis and U. travisiae from the marine polychaete Travisia forbesii were investigated with an emphasis on their general morphology and phylogenetic position. Solitary ovoid trophozoites and syzygies of U. ovalis were located free in the host coelom and showed metabolic activity, a non-progressive movement with periodic changes of the cell shape. Solitary trophozoites of U. travisiae, attached to the host tissue or free floating in the coelom, were V-shaped. Detached trophozoites demonstrated gliding motility, a progressive movement without observable cell body changes. In both gregarines, the cortex formed numerous epicytic folds, but superfolds appeared exclusively on the surface of U. ovalis during metabolic activity. SSU rDNA sequences obtained from U. ovalis and U. travisiae revealed that they belong to the Lecudinoidea clade; however, they are not affiliated with other coelomic urosporids (Pterospora spp. and Lithocystis spp.), but surprisingly with intestinal lecudinids (Difficilina spp.) parasitising nemerteans.

KW - 18S rDNA phylogeny

KW - Gliding and metaboly

KW - Marine eugregarines

KW - Superfolds

KW - Ultrastructure

KW - Urosporidae

UR - http://www.scopus.com/inward/record.url?scp=84970006305&partnerID=8YFLogxK

U2 - 10.1016/j.protis.2016.05.001

DO - 10.1016/j.protis.2016.05.001

M3 - Article

C2 - 27239726

VL - 167

SP - 279

EP - 301

JO - Protist

JF - Protist

SN - 1434-4610

IS - 3

ER -