TY - JOUR

T1 - Molecular mechanism of calcium-induced adsorption of DNA on zwitterionic phospholipid membranes

AU - Antipina, A.Y.

AU - Gurtovenko, A.A.

PY - 2015

Y1 - 2015

N2 - Interaction of DNA with zwitterionic phospholipids is an important long-standing problem in the field of liposome-based gene delivery. Although it is well-established that divalent cations can promote formation of stable DNA–phospholipid complexes, the underlying molecular mechanism remains largely unknown. Here we employ computer simulations to gain atomistically resolved insight into the kinetics of calcium-induced adsorption of DNA on zwitterionic phosphatidylcholine membranes as well as into the structure and stability of the resulting complexes. Overall, our findings show that calcium ions play a dual role in DNA–phospholipid systems. First, binding of divalent cations to the lipid–water interface turns the surface of the zwitterionic membrane positively charged, promoting thereby the initial electrostatic attraction of a polyanionic DNA molecule. Second, we show that calcium ions are crucial for stabilizing the DNA–lipid membrane complex as they bridge together phosphate groups of DNA and lipid molecule

AB - Interaction of DNA with zwitterionic phospholipids is an important long-standing problem in the field of liposome-based gene delivery. Although it is well-established that divalent cations can promote formation of stable DNA–phospholipid complexes, the underlying molecular mechanism remains largely unknown. Here we employ computer simulations to gain atomistically resolved insight into the kinetics of calcium-induced adsorption of DNA on zwitterionic phosphatidylcholine membranes as well as into the structure and stability of the resulting complexes. Overall, our findings show that calcium ions play a dual role in DNA–phospholipid systems. First, binding of divalent cations to the lipid–water interface turns the surface of the zwitterionic membrane positively charged, promoting thereby the initial electrostatic attraction of a polyanionic DNA molecule. Second, we show that calcium ions are crucial for stabilizing the DNA–lipid membrane complex as they bridge together phosphate groups of DNA and lipid molecule

U2 - 10.1021/acs.jpcb.5b01256

DO - 10.1021/acs.jpcb.5b01256

M3 - Article

VL - 119

SP - 6638

EP - 6645

JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

SN - 1520-6106

IS - 22

ER -