TY - JOUR

T1 - Molecular and structural analysis via hydrodynamic methods: Cationic poly(2-aminoethyl-methacrylate)s

AU - Perevyazko, Igor

AU - Trützschler, Anne K.

AU - Gubarev, Alexander

AU - Lebedeva, Elena

AU - Traeger, Anja

AU - Schubert, Ulrich S.

AU - Tsvetkov, Nikolay

PY - 2017/11/22

Y1 - 2017/11/22

N2 - Pharmaceutically relevant cationic linear poly(2-aminoethyl-methacrylate)s were synthesized by reversible addition-fragmentation chain transfer polymerization resulting in a homologous series of the homopolymers in a wide range of molar masses (10,000<M<130,000gmol−1) and relatively narrow dispersities (Ð:1.3±0.4). The polymers were studied in 0.2MNaCl and 0.2MNaOH in water, applying combined analytical approach comprising of complementary hydrodynamic and optical methods such as analytical ultracentrifugation, intrinsic viscosity, translation diffusion, flow birefringence and asymmetric flow field-flow fractionation (AF4) as well as standard characterization technique (SEC). The efforts were focused on the evaluation of solution (solvation), molecular (absolute molar masses), conformational (equilibrium rigidity, diameter of the polymer chain) and optical (anisotropy of optical polarizability of the monomer unit) characteristics of the polymers. Consequently the absolute values of the molar masses were determined by the sedimentation-diffusion analysis and AF4 resulting in well consistent results. The corresponding scaling relationships in 0.2MNaCl water ([η]=0.0031×M0.83; s0=0.0324×M0.39; D0=3,909×M−0.64) show linear trends in whole range of molar masses revealing random coil conformation of the macromolecules. Conformational characteristics were determined in 0.2MNaCl (equilibrium rigidity A=5.3±1.5nm) and 0.2MNaOH (A=1.3±0.1nm) revealing an influence of uncompensated charges on the polymer conformation.

AB - Pharmaceutically relevant cationic linear poly(2-aminoethyl-methacrylate)s were synthesized by reversible addition-fragmentation chain transfer polymerization resulting in a homologous series of the homopolymers in a wide range of molar masses (10,000<M<130,000gmol−1) and relatively narrow dispersities (Ð:1.3±0.4). The polymers were studied in 0.2MNaCl and 0.2MNaOH in water, applying combined analytical approach comprising of complementary hydrodynamic and optical methods such as analytical ultracentrifugation, intrinsic viscosity, translation diffusion, flow birefringence and asymmetric flow field-flow fractionation (AF4) as well as standard characterization technique (SEC). The efforts were focused on the evaluation of solution (solvation), molecular (absolute molar masses), conformational (equilibrium rigidity, diameter of the polymer chain) and optical (anisotropy of optical polarizability of the monomer unit) characteristics of the polymers. Consequently the absolute values of the molar masses were determined by the sedimentation-diffusion analysis and AF4 resulting in well consistent results. The corresponding scaling relationships in 0.2MNaCl water ([η]=0.0031×M0.83; s0=0.0324×M0.39; D0=3,909×M−0.64) show linear trends in whole range of molar masses revealing random coil conformation of the macromolecules. Conformational characteristics were determined in 0.2MNaCl (equilibrium rigidity A=5.3±1.5nm) and 0.2MNaOH (A=1.3±0.1nm) revealing an influence of uncompensated charges on the polymer conformation.

KW - Analytical ultracentrifugation

KW - Asymmetric flow field-flow fractionation

KW - Cationic polymers

KW - Characterization

KW - Poly(2-aminoethyl-methacrylate)

KW - Sedimentation

KW - Solution properties

KW - Viscosity

UR - http://www.scopus.com/inward/record.url?scp=85032450588&partnerID=8YFLogxK

U2 - 10.1016/j.polymer.2017.10.032

DO - 10.1016/j.polymer.2017.10.032

M3 - Article

AN - SCOPUS:85032450588

VL - 131

SP - 252

EP - 262

JO - Polymer

JF - Polymer

SN - 0032-3861

ER -