In this work, we proposed a rapid single-stage laser-induced fabrication of bimetallic micropatterns on the oxide glass surface using deep eutectic solvents (DESs) consisting of choline chloride, citric acid along with nickel, copper and cobalt acetates as metallization solutions. The resulting bimetallic micropatterns were tested as working electrodes for non-enzymatic determination of dopamine. The linear range for dopamine detection was found to be 1–500 µM, with sensitivity of 340.4 µA mM− 1 and 615.2 µA mM− 1 and detection limit of 0.36 µM and 0.51 µM for Ni-Cu and Ni-Co sensor, respectively. For the first time, bimetallic Ni-Cu and Ni-Co structures have been obtained from DESs for high-performance dopamine detection with great potential for further application in non-enzymatic sensing and biosensing.