We observed the bright supergiant ρ Leo (B1 lab) in January-February 2004 using the 6-m telescope of the Special Astrophysical Observatory (Russia) and the 1.8-m telescope of the Bohyunsan Optical Astronomy Observatory (South Korea). 47 spectra with high time resolution (4-10 min), signal-to-noise ratios 300-1000, and spectral resolutions 45 000-60 000 were obtained. We detected variability in the HeI, SiII, SiIII, and NII line profiles, which may be due to rotational modulation of the profiles and photospheric pulsations of ρ Leo. The possible influence of the stellar magnetic field on the line-profile variations is discussed.