Micronutrient (Fe, Mn, Zn) and Ca distribution in Zea mays L. kernels during their germination in water was studied by the method of X-ray fluorescent analysis. The greatest nutrient translocation from the kernel to the axial organs took place immediately after radicle emergence (about 30 h). A growth-dependent increase in the content of Ca and Zn (μg/shoot) exceeded their increase in the root, whereas as regards Mn and Fe, the two organs were almost alike. During kernel imbibition (before the radicle emerged), all these nutrients first leaked partially into the medium and then were reabsorbed (except for Mn) by the seedling. The rates of nutrient translocation from the scutellum and endosperm were different. Within 120 h following the onset of the experiment, the Ca and Mn export was observed only from the endosperm, while their content in the scutellum did not change significantly. While the Fe content in the endosperm was declining during the whole experimental period, in the scutellum, it dropped mainly after 72 h. Essential Zn export from the endosperm was observed only after 72 h, whereas Zn export from the scutellum commenced during the first day of kernel imbibition, probably due to Zn leaking through the seed coat into the medium. As the kernel germinated, the endosperm contribution to the development of the total pool of mobile nutrients increased. The endosperm was the main source of Ca, Fe, and Mn for the axial organs, whereas the Zn supply came from both the endosperm and the scutellum.