Methyl-β-cyclodextrin modulates thapsigargin-induced store-dependent Ca2+ entry in macrophages

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Methyl-β-cyclodextrin modulates thapsigargin-induced store-dependent Ca2+ entry in macrophages. / Krutetskaya, Z.I.; Milenina, L.S.; Naumova, A.A.; Butov, S.N.; Antonov, V.G.; Nozdrachev, A.D.

в: Doklady Biochemistry and Biophysics, Том 473, № 1, 2017, стр. 88-90.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{c6d94213cb6e49d2901efccdc5aa0fb6,

title = "Methyl-β-cyclodextrin modulates thapsigargin-induced store-dependent Ca2+ entry in macrophages",

abstract = "Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with methyl-β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, leads to significant inhibition of thapsigargin-induced store-dependent Ca2+ entry in rat peritoneal macrophages. In contrast, macrophage treatment with methyl-β-cyclodextrin after Ca2+ entry mechanisms were activated by store depletion by thapsigargin application leads to potentiation of subsequent store-dependent Ca2+ entry. The results suggest that intact lipid rafts are necessary for the activation but not the maintenance of store-dependent Ca2+ entry in macrophages.",

author = "Z.I. Krutetskaya and L.S. Milenina and A.A. Naumova and S.N. Butov and V.G. Antonov and A.D. Nozdrachev",

note = "Krutetskaya, Z.I., Milenina, L.S., Naumova, A.A. et al. Methyl-β-cyclodextrin modulates thapsigargin-induced store-dependent Ca2+ entry in macrophages. Dokl Biochem Biophys 473, 88–90 (2017). https://doi.org/10.1134/S1607672917020028",

year = "2017",

language = "English",

volume = "473",

pages = "88--90",

journal = "Doklady Biochemistry and Biophysics",

issn = "1607-6729",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "1",

}

RIS

TY - JOUR

T1 - Methyl-β-cyclodextrin modulates thapsigargin-induced store-dependent Ca2+ entry in macrophages

AU - Krutetskaya, Z.I.

AU - Milenina, L.S.

AU - Naumova, A.A.

AU - Butov, S.N.

AU - Antonov, V.G.

AU - Nozdrachev, A.D.

N1 - Krutetskaya, Z.I., Milenina, L.S., Naumova, A.A. et al. Methyl-β-cyclodextrin modulates thapsigargin-induced store-dependent Ca2+ entry in macrophages. Dokl Biochem Biophys 473, 88–90 (2017). https://doi.org/10.1134/S1607672917020028

PY - 2017

Y1 - 2017

N2 - Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with methyl-β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, leads to significant inhibition of thapsigargin-induced store-dependent Ca2+ entry in rat peritoneal macrophages. In contrast, macrophage treatment with methyl-β-cyclodextrin after Ca2+ entry mechanisms were activated by store depletion by thapsigargin application leads to potentiation of subsequent store-dependent Ca2+ entry. The results suggest that intact lipid rafts are necessary for the activation but not the maintenance of store-dependent Ca2+ entry in macrophages.

AB - Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with methyl-β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, leads to significant inhibition of thapsigargin-induced store-dependent Ca2+ entry in rat peritoneal macrophages. In contrast, macrophage treatment with methyl-β-cyclodextrin after Ca2+ entry mechanisms were activated by store depletion by thapsigargin application leads to potentiation of subsequent store-dependent Ca2+ entry. The results suggest that intact lipid rafts are necessary for the activation but not the maintenance of store-dependent Ca2+ entry in macrophages.

UR - https://link.springer.com/article/10.1134/S1607672917020028

M3 - Article

VL - 473

SP - 88

EP - 90

JO - Doklady Biochemistry and Biophysics

JF - Doklady Biochemistry and Biophysics

SN - 1607-6729

IS - 1

ER -

ID: 9237455