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Methods for acquisition of quantitative data from confocal images of gene expression in situ. / Surkova, S. Yu; Myasnikova, E. M.; Kozlov, K. N.; Samsonova, A. A.; Reinitz, J.; Samsonova, M. G.

в: Cell and Tissue Biology, Том 2, № 2, 01.04.2008, стр. 200-215.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Surkova, SY, Myasnikova, EM, Kozlov, KN, Samsonova, AA, Reinitz, J & Samsonova, MG 2008, 'Methods for acquisition of quantitative data from confocal images of gene expression in situ', Cell and Tissue Biology, Том. 2, № 2, стр. 200-215. https://doi.org/10.1134/S1990519X08020156

APA

Surkova, S. Y., Myasnikova, E. M., Kozlov, K. N., Samsonova, A. A., Reinitz, J., & Samsonova, M. G. (2008). Methods for acquisition of quantitative data from confocal images of gene expression in situ. Cell and Tissue Biology, 2(2), 200-215. https://doi.org/10.1134/S1990519X08020156

Vancouver

Surkova SY, Myasnikova EM, Kozlov KN, Samsonova AA, Reinitz J, Samsonova MG. Methods for acquisition of quantitative data from confocal images of gene expression in situ. Cell and Tissue Biology. 2008 Апр. 1;2(2):200-215. https://doi.org/10.1134/S1990519X08020156

Author

Surkova, S. Yu ; Myasnikova, E. M. ; Kozlov, K. N. ; Samsonova, A. A. ; Reinitz, J. ; Samsonova, M. G. / Methods for acquisition of quantitative data from confocal images of gene expression in situ. в: Cell and Tissue Biology. 2008 ; Том 2, № 2. стр. 200-215.

BibTeX

@article{1069e70218474c5cb999d785d7597243,
title = "Methods for acquisition of quantitative data from confocal images of gene expression in situ",
abstract = "In this review, we summarize original methods for the extraction of quantitative information from confocal images of gene-expression patterns. These methods include image segmentation, the extraction of quantitative numerical data on gene expression, and the removal of background signal and spatial registration. Finally, it is possible to construct a spatiotemporal atlas of gene expression from individual images recorded at each developmental stage. Initially all methods were developed to extract quantitative numerical information from confocal images of segmentation gene expression in Drosophila melanogaster. The application of these methods to Drosophila images makes it possible to reveal new mechanisms in the formation of segmentation gene expression domains, as well as to construct a quantitative atlas of segmentation gene expression. Most image processing procedures can be easily adapted to process a wide range of biological images.",
keywords = "Background removal, Confocal microscopy, Image segmentation, Methods of image processing, Quantitative gene expression, Spatial registration",
author = "Surkova, {S. Yu} and Myasnikova, {E. M.} and Kozlov, {K. N.} and Samsonova, {A. A.} and J. Reinitz and Samsonova, {M. G.}",
year = "2008",
month = apr,
day = "1",
doi = "10.1134/S1990519X08020156",
language = "English",
volume = "2",
pages = "200--215",
journal = "Cell and Tissue Biology",
issn = "1990-519X",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "2",

}

RIS

TY - JOUR

T1 - Methods for acquisition of quantitative data from confocal images of gene expression in situ

AU - Surkova, S. Yu

AU - Myasnikova, E. M.

AU - Kozlov, K. N.

AU - Samsonova, A. A.

AU - Reinitz, J.

AU - Samsonova, M. G.

PY - 2008/4/1

Y1 - 2008/4/1

N2 - In this review, we summarize original methods for the extraction of quantitative information from confocal images of gene-expression patterns. These methods include image segmentation, the extraction of quantitative numerical data on gene expression, and the removal of background signal and spatial registration. Finally, it is possible to construct a spatiotemporal atlas of gene expression from individual images recorded at each developmental stage. Initially all methods were developed to extract quantitative numerical information from confocal images of segmentation gene expression in Drosophila melanogaster. The application of these methods to Drosophila images makes it possible to reveal new mechanisms in the formation of segmentation gene expression domains, as well as to construct a quantitative atlas of segmentation gene expression. Most image processing procedures can be easily adapted to process a wide range of biological images.

AB - In this review, we summarize original methods for the extraction of quantitative information from confocal images of gene-expression patterns. These methods include image segmentation, the extraction of quantitative numerical data on gene expression, and the removal of background signal and spatial registration. Finally, it is possible to construct a spatiotemporal atlas of gene expression from individual images recorded at each developmental stage. Initially all methods were developed to extract quantitative numerical information from confocal images of segmentation gene expression in Drosophila melanogaster. The application of these methods to Drosophila images makes it possible to reveal new mechanisms in the formation of segmentation gene expression domains, as well as to construct a quantitative atlas of segmentation gene expression. Most image processing procedures can be easily adapted to process a wide range of biological images.

KW - Background removal

KW - Confocal microscopy

KW - Image segmentation

KW - Methods of image processing

KW - Quantitative gene expression

KW - Spatial registration

UR - http://www.scopus.com/inward/record.url?scp=43149103647&partnerID=8YFLogxK

U2 - 10.1134/S1990519X08020156

DO - 10.1134/S1990519X08020156

M3 - Article

C2 - 18664119

AN - SCOPUS:43149103647

VL - 2

SP - 200

EP - 215

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 2

ER -

ID: 34107508