Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Long-read sequencing technologies have substantially improved the assemblies of many isolate bacterial genomes as compared to fragmented short-read assemblies. However, assembling complex metagenomic datasets remains difficult even for state-of-the-art long-read assemblers. Here we present metaFlye, which addresses important long-read metagenomic assembly challenges, such as uneven bacterial composition and intra-species heterogeneity. First, we benchmarked metaFlye using simulated and mock bacterial communities and show that it consistently produces assemblies with better completeness and contiguity than state-of-the-art long-read assemblers. Second, we performed long-read sequencing of the sheep microbiome and applied metaFlye to reconstruct 63 complete or nearly complete bacterial genomes within single contigs. Finally, we show that long-read assembly of human microbiomes enables the discovery of full-length biosynthetic gene clusters that encode biomedically important natural products.
Язык оригинала | английский |
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Страницы (с-по) | 1103-1110 |
Число страниц | 8 |
Журнал | Nature Methods |
Том | 17 |
Номер выпуска | 11 |
Дата раннего онлайн-доступа | 5 окт 2020 |
DOI | |
Состояние | Опубликовано - 1 ноя 2020 |
ID: 62913419