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DOI

Long-read sequencing technologies have substantially improved the assemblies of many isolate bacterial genomes as compared to fragmented short-read assemblies. However, assembling complex metagenomic datasets remains difficult even for state-of-the-art long-read assemblers. Here we present metaFlye, which addresses important long-read metagenomic assembly challenges, such as uneven bacterial composition and intra-species heterogeneity. First, we benchmarked metaFlye using simulated and mock bacterial communities and show that it consistently produces assemblies with better completeness and contiguity than state-of-the-art long-read assemblers. Second, we performed long-read sequencing of the sheep microbiome and applied metaFlye to reconstruct 63 complete or nearly complete bacterial genomes within single contigs. Finally, we show that long-read assembly of human microbiomes enables the discovery of full-length biosynthetic gene clusters that encode biomedically important natural products.

Язык оригиналаанглийский
Страницы (с-по)1103-1110
Число страниц8
ЖурналNature Methods
Том17
Номер выпуска11
Дата раннего онлайн-доступа5 окт 2020
DOI
СостояниеОпубликовано - 1 ноя 2020

    Предметные области Scopus

  • Молекулярная биология
  • Биохимия
  • Биотехнология
  • Клеточная биология

ID: 62913419