Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Metabolic Fingerprints from the Human Oral Microbiome Reveal a Vast Knowledge Gap of Secreted Small Peptidic Molecules. / Edlund, Anna; Garg, Neha; Mohimani, Hosein; Gurevich, Alexey; He, Xuesong; Shi, Wenyuan; Dorrestein, Pieter C.; McLean, Jeffrey S.
в: mSystems, Том 2, № 4, e00058, 29.08.2017.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Metabolic Fingerprints from the Human Oral Microbiome Reveal a Vast Knowledge Gap of Secreted Small Peptidic Molecules
AU - Edlund, Anna
AU - Garg, Neha
AU - Mohimani, Hosein
AU - Gurevich, Alexey
AU - He, Xuesong
AU - Shi, Wenyuan
AU - Dorrestein, Pieter C.
AU - McLean, Jeffrey S.
PY - 2017/8/29
Y1 - 2017/8/29
N2 - Recent research indicates that the human microbiota play key roles in maintaining health by providing essential nutrients, providing immune education, and preventing pathogen expansion. Processes underlying the transition from a healthy human microbiome to a disease-associated microbiome are poorly understood, partially because of the potential influences from a wide diversity of bacterium-derived compounds that are illy defined. Here, we present the analysis of peptidic small molecules (SMs) secreted from bacteria and viewed from a temporal perspective. Through comparative analysis of mass spectral profiles from a collection of cultured oral isolates and an established in vitro multispecies oral community, we found that the production of SMs both delineates a temporal expression pattern and allows discrimination between bacterial isolates at the species level. Importantly, the majority of the identified molecules were of unknown identity, and only ∼2.2% could be annotated and classified. The catalogue of bacterially produced SMs we obtained in this study reveals an undiscovered molecular world for which compound isolation and ecosystem testing will facilitate a better understanding of their roles in human health and disease.
AB - Recent research indicates that the human microbiota play key roles in maintaining health by providing essential nutrients, providing immune education, and preventing pathogen expansion. Processes underlying the transition from a healthy human microbiome to a disease-associated microbiome are poorly understood, partially because of the potential influences from a wide diversity of bacterium-derived compounds that are illy defined. Here, we present the analysis of peptidic small molecules (SMs) secreted from bacteria and viewed from a temporal perspective. Through comparative analysis of mass spectral profiles from a collection of cultured oral isolates and an established in vitro multispecies oral community, we found that the production of SMs both delineates a temporal expression pattern and allows discrimination between bacterial isolates at the species level. Importantly, the majority of the identified molecules were of unknown identity, and only ∼2.2% could be annotated and classified. The catalogue of bacterially produced SMs we obtained in this study reveals an undiscovered molecular world for which compound isolation and ecosystem testing will facilitate a better understanding of their roles in human health and disease.
KW - Biofilms
KW - Lactobacillus
KW - Oral microbiology
KW - Peptidic small molecules
KW - Streptococcus
KW - Veillonella
UR - http://www.scopus.com/inward/record.url?scp=85041537615&partnerID=8YFLogxK
U2 - 10.1128/mSystems.00058-17
DO - 10.1128/mSystems.00058-17
M3 - Article
AN - SCOPUS:85041537615
VL - 2
JO - Living Reviews in Solar Physics
JF - Living Reviews in Solar Physics
SN - 2367-3648
IS - 4
M1 - e00058
ER -
ID: 33139388