TY - JOUR

T1 - Mesoglein Expression During Aurelia aurita Life Cycle

AU - Kotova, Anastasiya

AU - Naiden, Anastasiya

AU - Shumeev, Alexander

AU - Shaposhnikova, Tatiana

AU - Podgornaya, Olga

AU - Adonin, Leonid

PY - 2016

Y1 - 2016

N2 - Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by the layer of extracellular matrix (ECM) called mesoglea. Aurelia aurita complex life cycle consists of several stages including alternating generations of sexual adult stage medusa and asexual stage polyp (scyphistoma). The main difference between polyp and medusa is the degree of the ECM (mesoglea) development. The new protein “mesoglein” was determined as one of the main components of mesoglea. Mesoglein is the component of the mesoglea “elastic” fibers. Previously we found that according to reverse transcription PCR mesoglein is expressed in the mature medusa exclusively in the mesogleal cells. The aim of the current work was to find out at what stage of development the specific mesoglein expression occurs. Mesogleins’ expression have been checked by PCR with specific primers on the template of transcriptomes’ cDNA from different stages; by mesogleins’ antibody staining on SDSPAGE and on paraffin s

AB - Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by the layer of extracellular matrix (ECM) called mesoglea. Aurelia aurita complex life cycle consists of several stages including alternating generations of sexual adult stage medusa and asexual stage polyp (scyphistoma). The main difference between polyp and medusa is the degree of the ECM (mesoglea) development. The new protein “mesoglein” was determined as one of the main components of mesoglea. Mesoglein is the component of the mesoglea “elastic” fibers. Previously we found that according to reverse transcription PCR mesoglein is expressed in the mature medusa exclusively in the mesogleal cells. The aim of the current work was to find out at what stage of development the specific mesoglein expression occurs. Mesogleins’ expression have been checked by PCR with specific primers on the template of transcriptomes’ cDNA from different stages; by mesogleins’ antibody staining on SDSPAGE and on paraffin s

KW - Aurelia aurita

KW - Semaeostomeae

KW - Ulmaridae

KW - Scyphozoa

KW - Mesoglea

U2 - 10.4172/2155-9910.1000211

DO - 10.4172/2155-9910.1000211

M3 - Article

VL - 6

SP - 1

EP - 7

JO - Journal of Marine Science: Research & Development

JF - Journal of Marine Science: Research & Development

SN - 2155-9910

IS - 6

ER -