This study focuses on the mechanisms underlying the formation of the primary animal-vegetal axis in annelid development and its evolutionary transformations. Through a comparative analysis of morphological, molecular-genetic, and experimental data, we demonstrated that the specification of cell lineages and body axes arises from the interplay of autonomous mechanisms (e.g., ooplasmic segregation, maternal determinants) and conditional mechanisms (e.g., intercellular signaling, zygotic genome activation). Cleavage asymmetry, regulated by cytoskeletal dynamics and β-catenin localization, is shown to depend on the localization of the meiotic spindle and plays a pivotal role in the primary polarization of the embryo. The causes and consequences of evolutionary changes in annelid development are analyzed in detail. Notably, the transition from homoquadrant (equal) to heteroquadrant (unequal) cleavage is accompanied by enhanced autonomous specification, accelerated zygotic transcription, and reduction of larval structures. Experimental modulation of Wnt and MAPK signaling pathways in the polychaete annelid Ophelia limacina confirms the conserved role of intercellular signaling in defining vegetal and posterodorsal territories. A hypothesis is proposed regarding the co-option of mechanisms originally responsible for animal-vegetal axis polarization to generate asymmetry between quadrants. Additionally, heterochrony and heterotopy are identified as key drivers of evolutionary diversity in annelids.