Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Mapping epigenetic modifications on chicken lampbrush chromosomes. / Kulikova, Tatiana; Surkova, Anna; Zlotina, Anna; Krasikova, Alla.
в: Molecular Cytogenetics, Том 13, № 1, 32, 03.08.2020, стр. 1-15.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Mapping epigenetic modifications on chicken lampbrush chromosomes
AU - Kulikova, Tatiana
AU - Surkova, Anna
AU - Zlotina, Anna
AU - Krasikova, Alla
N1 - Funding Information: This work was supported by the Russian Science Foundation (grant no. 19– 74-20075). Publisher Copyright: © 2020 The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/8/3
Y1 - 2020/8/3
N2 - Background: The epigenetic regulation of genome is crucial for implementation of the genetic program of ontogenesis through establishing and maintaining differential gene expression. Thus mapping of various epigenetic modifications to the genome is relevant for studying the regulation of gene expression. Giant transcriptionally active lampbrush chromosomes are an established tool for high resolution physical mapping of the genome and its epigenetic modifications. This study is aimed at characterizing the epigenetic status of compact chromatin domains (chromomeres) of chicken lampbrush macrochromosomes. Results: Distribution of three epigenetic modifications - 5-methylcytosine, histone H3 trimethylated at lysine 9 and hyperacetylated histone H4 - along the axes of chicken lampbrush chromosomes 1-4, Z and W was analyzed in details. Enrichment of chromatin domains with the investigated epigenetic modifications was indicated on the cytological chromomere-loop maps for corresponding chicken lampbrush chromosomes. Heterogeneity in the distribution of 5-methylcytosine and histone H3 trimethylated at lysine 9 along the chromosome axes was revealed. Conclusions: On examples of certain chromomeres of chicken lampbrush chromosomes 1, 3, 4 and W we demonstrated that a combination of immunofluorescent staining and fluorescence in situ hybridization allows to relate the epigenetic status and a DNA sequence context of individual chromomeres.
AB - Background: The epigenetic regulation of genome is crucial for implementation of the genetic program of ontogenesis through establishing and maintaining differential gene expression. Thus mapping of various epigenetic modifications to the genome is relevant for studying the regulation of gene expression. Giant transcriptionally active lampbrush chromosomes are an established tool for high resolution physical mapping of the genome and its epigenetic modifications. This study is aimed at characterizing the epigenetic status of compact chromatin domains (chromomeres) of chicken lampbrush macrochromosomes. Results: Distribution of three epigenetic modifications - 5-methylcytosine, histone H3 trimethylated at lysine 9 and hyperacetylated histone H4 - along the axes of chicken lampbrush chromosomes 1-4, Z and W was analyzed in details. Enrichment of chromatin domains with the investigated epigenetic modifications was indicated on the cytological chromomere-loop maps for corresponding chicken lampbrush chromosomes. Heterogeneity in the distribution of 5-methylcytosine and histone H3 trimethylated at lysine 9 along the chromosome axes was revealed. Conclusions: On examples of certain chromomeres of chicken lampbrush chromosomes 1, 3, 4 and W we demonstrated that a combination of immunofluorescent staining and fluorescence in situ hybridization allows to relate the epigenetic status and a DNA sequence context of individual chromomeres.
KW - Chicken
KW - Chromatin domain
KW - Chromomere
KW - Cytological chromomere-loop map
KW - Epigenetic modifications
KW - Gene mapping
KW - Histone modifications
KW - Karyotype
KW - Lampbrush chromosomes
KW - Methylated cytosine
KW - Tandem repeats
UR - http://www.scopus.com/inward/record.url?scp=85089382033&partnerID=8YFLogxK
U2 - 10.1186/s13039-020-00496-0
DO - 10.1186/s13039-020-00496-0
M3 - Article
AN - SCOPUS:85089382033
VL - 13
SP - 1
EP - 15
JO - Molecular Cytogenetics
JF - Molecular Cytogenetics
SN - 1755-8166
IS - 1
M1 - 32
ER -
ID: 71917024