Manganese availability can be a serious limiting factor for plant growth. Trans-location of manganese (Mn) in the tissues of plants from the families Gramíne-ae, Brassicáceae and Leguminósae (L.)was studied on a strongly acidic Albic Re-tisols limed with increasing doses of conversion chalk (CC) in a 5-year pot ex-periment. Studied crops were vetch, rapeseed and barley. Results showed that the transfer of Mn into plants decreases with the increasing dose of CC. There was a correlation between the manganese content in the grain and straw of spring barley (r = 0.96; P < 0.01). The concentration of Mn in the tissues of barley and rapeseed fits into the range capable for ensuring the proper functioning of plants. The doses of conversion chalk above 13.6 t ha–1 led to a decrease in the uptake of Mn by vetch to the lower deficiency threshold. The results imply that the role of Mn for plants is still largely underestimated and requires further study.