Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Lysine-based dendrimer with double arginine residues. / Sheveleva, Nadezhda N.; Markelov, Denis A.; Vovk, Mikhail A.; Mikhailova, Mariya E.; Tarasenko, Irina I.; Tolstoy, Peter M.; Neelov, Igor M.; Lähderanta, Erkki.
в: RSC Advances, Том 9, № 31, 09.06.2019, стр. 18018-18026.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Lysine-based dendrimer with double arginine residues
AU - Sheveleva, Nadezhda N.
AU - Markelov, Denis A.
AU - Vovk, Mikhail A.
AU - Mikhailova, Mariya E.
AU - Tarasenko, Irina I.
AU - Tolstoy, Peter M.
AU - Neelov, Igor M.
AU - Lähderanta, Erkki
PY - 2019/6/9
Y1 - 2019/6/9
N2 - Due to their well-defined structure, multivalency, biocompatibility, and low toxicity, lysine dendrimers can be used as safe and efficient nanocarriers for drug and gene delivery. One useful strategy for improving the gene delivery properties of dendrimers is modification with arginine amino acid (Arg) residues. Incorporation of Arg residues could be favorable for the enhancement in transfection efficiency of lysine based dendrimers. In this work, we have synthesized a new second-generation poly-l-lysine dendrimer with repeating units containing two linear Arg residues between neighboring lysine branching points (Lys-2Arg dendrimer) and studied its physicochemical properties. We confirmed the structure of Lys-2Arg dendrimer using various one- and two-dimensional 1H and 13C NMR spectroscopy methods. Comparison of T1H relaxation data for Lys-2Arg and Lys-2Lys dendrimers showed that the replacement of double Lys residues with double Arg residues resulted in a sharp decrease in the mobility of methylene groups in side segments and in the main chain of ϵ-Lys inner segments. We suggest that this unexpected effect is caused by a guanidine-guanidine pairing effect in water, which leads to entanglements between dendrimer branches.
AB - Due to their well-defined structure, multivalency, biocompatibility, and low toxicity, lysine dendrimers can be used as safe and efficient nanocarriers for drug and gene delivery. One useful strategy for improving the gene delivery properties of dendrimers is modification with arginine amino acid (Arg) residues. Incorporation of Arg residues could be favorable for the enhancement in transfection efficiency of lysine based dendrimers. In this work, we have synthesized a new second-generation poly-l-lysine dendrimer with repeating units containing two linear Arg residues between neighboring lysine branching points (Lys-2Arg dendrimer) and studied its physicochemical properties. We confirmed the structure of Lys-2Arg dendrimer using various one- and two-dimensional 1H and 13C NMR spectroscopy methods. Comparison of T1H relaxation data for Lys-2Arg and Lys-2Lys dendrimers showed that the replacement of double Lys residues with double Arg residues resulted in a sharp decrease in the mobility of methylene groups in side segments and in the main chain of ϵ-Lys inner segments. We suggest that this unexpected effect is caused by a guanidine-guanidine pairing effect in water, which leads to entanglements between dendrimer branches.
KW - LATTICE NMR RELAXATION
KW - PEPTIDE DENDRIMERS
KW - PAMAM DENDRIMER
KW - SIRNA DELIVERY
KW - DYNAMICS
KW - TRANSFECTION
KW - MANIFESTATION
KW - SIMULATION
UR - http://www.scopus.com/inward/record.url?scp=85067427858&partnerID=8YFLogxK
U2 - 10.1039/c9ra02461a
DO - 10.1039/c9ra02461a
M3 - Article
AN - SCOPUS:85067427858
VL - 9
SP - 18018
EP - 18026
JO - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 31
ER -
ID: 43525753