Liquid–Liquid Microextraction of Tetracyclines from Biological Fluids for Their Subsequent Determination by High-Performance Liquid Chromatography with UV Detection

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Liquid–Liquid Microextraction of Tetracyclines from Biological Fluids for Their Subsequent Determination by High-Performance Liquid Chromatography with UV Detection. / Cherkashina, K. D.; Sumina, A. I.; Vakh, K. S.; Bulatov, A. V.

в: Journal of Analytical Chemistry, Том 75, № 11, 11.2020, стр. 1424-1429.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{e8ddb9d70e5540cda2613bd2f43df55e,

title = "Liquid–Liquid Microextraction of Tetracyclines from Biological Fluids for Their Subsequent Determination by High-Performance Liquid Chromatography with UV Detection",

abstract = "Abstract: We proposed a liquid–liquid microextraction procedure for the isolation and preconcentration of tetracycline antibiotics from biological fluids. The procedure involves in situ formation of a micellar phase of the primary amine from an aqueous sample solution with the introduction of a polar organic solvent. Possibilities of the developed procedures are demonstrated on an example of the determination of tetracycline, oxytetracycline, and doxycycline in human plasma, blood serum, and urine by HPLC–UV. The limits of detection (3σ) for oxytetracycline and doxycycline were 0.03 mg/L, for tetracycline, it was 0.08 mg/L. The extraction time does not exceed 5 min.",

keywords = "biological fluids, high-performance liquid chromatography, liquid–liquid microextraction, tetracyclines",

author = "Cherkashina, {K. D.} and Sumina, {A. I.} and Vakh, {K. S.} and Bulatov, {A. V.}",

note = "Publisher Copyright: {\textcopyright} 2020, Pleiades Publishing, Ltd.",

year = "2020",

month = nov,

doi = "10.1134/S1061934820090075",

language = "English",

volume = "75",

pages = "1424--1429",

journal = "Journal of Analytical Chemistry",

issn = "1061-9348",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "11",

}

RIS

TY - JOUR

T1 - Liquid–Liquid Microextraction of Tetracyclines from Biological Fluids for Their Subsequent Determination by High-Performance Liquid Chromatography with UV Detection

AU - Cherkashina, K. D.

AU - Sumina, A. I.

AU - Vakh, K. S.

AU - Bulatov, A. V.

PY - 2020/11

Y1 - 2020/11

N2 - Abstract: We proposed a liquid–liquid microextraction procedure for the isolation and preconcentration of tetracycline antibiotics from biological fluids. The procedure involves in situ formation of a micellar phase of the primary amine from an aqueous sample solution with the introduction of a polar organic solvent. Possibilities of the developed procedures are demonstrated on an example of the determination of tetracycline, oxytetracycline, and doxycycline in human plasma, blood serum, and urine by HPLC–UV. The limits of detection (3σ) for oxytetracycline and doxycycline were 0.03 mg/L, for tetracycline, it was 0.08 mg/L. The extraction time does not exceed 5 min.

AB - Abstract: We proposed a liquid–liquid microextraction procedure for the isolation and preconcentration of tetracycline antibiotics from biological fluids. The procedure involves in situ formation of a micellar phase of the primary amine from an aqueous sample solution with the introduction of a polar organic solvent. Possibilities of the developed procedures are demonstrated on an example of the determination of tetracycline, oxytetracycline, and doxycycline in human plasma, blood serum, and urine by HPLC–UV. The limits of detection (3σ) for oxytetracycline and doxycycline were 0.03 mg/L, for tetracycline, it was 0.08 mg/L. The extraction time does not exceed 5 min.

KW - biological fluids

KW - high-performance liquid chromatography

KW - liquid–liquid microextraction

KW - tetracyclines

UR - http://www.scopus.com/inward/record.url?scp=85097793733&partnerID=8YFLogxK

U2 - 10.1134/S1061934820090075

DO - 10.1134/S1061934820090075

M3 - Article

AN - SCOPUS:85097793733

VL - 75

SP - 1424

EP - 1429

JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1061-9348

IS - 11

ER -

ID: 93273188