The lichen checklist of the former manors in the Smolensk Region of Russia is presented. It comprises 139 species, including 1 lichenicolous lichen and 4 non-lichenized calicioid fungi. Arthonia didyma, Arthrosporum populorum, Athallia pyracea, Bacidina arnoldiana, Calicium glaucellum, Calogaya decipiens, Candelaria pacifica, Candelariella lutella, Chaenotheca furfuracea, Coenogonium pineti, Eopyrenula leucoplaca, Lecania erysibe, Lecanora compallens, L. expallens, L. umbrina, Melanohalea septentrionalis, Myriolecis hagenii, M. perpruinosa, Parmelina tiliacea, Placynthiella dasaea, P. icmalea, Ramalina europaea, Rinodina sophodes, R. subparieta, Scutula dedicata, Strangospora pinicola, and Trapeliopsis flexuosa are new to the Smolensk Region.