TY - JOUR

T1 - Large Extracellular Vesicles Derived from Natural Killer Cells Affect the Functions of Monocytes.

AU - Соколов, Дмитрий

AU - Горшкова, Алина Алексеевна

AU - Тыщук, Елизавета Владимировна

AU - Гребенкина, Полина Владимировна

AU - Зементова, Мария Сергеевна

AU - Коган, Игорь Юрьевич

AU - Тотолян, Арег А.

PY - 2024/8/31

Y1 - 2024/8/31

N2 - Communication between natural killer cells (NK cells) and monocytes/macrophages may play an important role in immunomodulation and regulation of inflammatory processes. The aim of this research was to investigate the impact of NK cell-derived large extracellular vesicles on monocyte function because this field is understudied. We studied how NK-cell derived large extracellular vesicles impact on THP-1 cells characteristics after coculturing: phenotype, functions were observed with flow cytometry. In this study, we demonstrated the ability of large extracellular vesicles produced by NK cells to integrate into the membranes of THP-1 cells and influence the viability, phenotype, and functional characteristics of the cells. The results obtained demonstrate the ability of large extracellular vesicles to act as an additional component in the immunomodulatory activity of NK cells in relation to monocytes.

AB - Communication between natural killer cells (NK cells) and monocytes/macrophages may play an important role in immunomodulation and regulation of inflammatory processes. The aim of this research was to investigate the impact of NK cell-derived large extracellular vesicles on monocyte function because this field is understudied. We studied how NK-cell derived large extracellular vesicles impact on THP-1 cells characteristics after coculturing: phenotype, functions were observed with flow cytometry. In this study, we demonstrated the ability of large extracellular vesicles produced by NK cells to integrate into the membranes of THP-1 cells and influence the viability, phenotype, and functional characteristics of the cells. The results obtained demonstrate the ability of large extracellular vesicles to act as an additional component in the immunomodulatory activity of NK cells in relation to monocytes.

KW - Cell Communication/immunology

KW - Cell Survival

KW - Coculture Techniques

KW - Extracellular Vesicles/immunology

KW - Humans

KW - Killer Cells, Natural/immunology

KW - Macrophages/immunology

KW - Monocytes/immunology

KW - THP-1 Cells

KW - THP-1

KW - monocytes

KW - NK-92

KW - large extracellular vesicles

KW - PBMC

KW - natural killers

KW - ectosomes

UR - https://www.mendeley.com/catalogue/edfe0d88-ba46-3de3-b0c8-c3e1fcd85fce/

U2 - 10.3390/ijms25179478

DO - 10.3390/ijms25179478

M3 - Article

C2 - 39273424

VL - 25

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 17

M1 - 9478

ER -