Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Lactoferrin from canine neutrophils : Isolation and physicochemical and antimicrobial properties. / Berlov, M. N.; Korableva, E. S.; Andreeva, Yu V.; Ovchinnikova, T. V.; Kokryakov, V. N.
в: Biochemistry (Moscow), Том 72, № 4, 04.2007, стр. 445-451.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Lactoferrin from canine neutrophils
T2 - Isolation and physicochemical and antimicrobial properties
AU - Berlov, M. N.
AU - Korableva, E. S.
AU - Andreeva, Yu V.
AU - Ovchinnikova, T. V.
AU - Kokryakov, V. N.
N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research (grant Nos. 03 04 49349 and 06 04 49416).
PY - 2007/4
Y1 - 2007/4
N2 - Lactoferrin has been isolated from canine leukocytes for the first time. Lactoferrin was identified by N-terminal amino acid sequence and by capability to capture ferric cations resulting in a complex with absorbance maximum at 460-470 nm. It is demonstrated that canine lactoferrin resembles the human homolog in some physicochemical properties, i.e. molecular weight, carbohydrate presence, and conditions of protein-iron complex dissociation. Bactericidal activity of dog lactoferrin was demonstrated on the gram-negative bacterium Escherichia coli and gram-positive bacterium Listeria monocytogenes. Bactericidal activity of canine lactoferrin is similar to that of human lactoferrin.
AB - Lactoferrin has been isolated from canine leukocytes for the first time. Lactoferrin was identified by N-terminal amino acid sequence and by capability to capture ferric cations resulting in a complex with absorbance maximum at 460-470 nm. It is demonstrated that canine lactoferrin resembles the human homolog in some physicochemical properties, i.e. molecular weight, carbohydrate presence, and conditions of protein-iron complex dissociation. Bactericidal activity of dog lactoferrin was demonstrated on the gram-negative bacterium Escherichia coli and gram-positive bacterium Listeria monocytogenes. Bactericidal activity of canine lactoferrin is similar to that of human lactoferrin.
KW - Antimicrobial activity
KW - Dog
KW - Lactoferrin
KW - Neutrophil
UR - http://www.scopus.com/inward/record.url?scp=34247369794&partnerID=8YFLogxK
U2 - 10.1134/S0006297907040128
DO - 10.1134/S0006297907040128
M3 - Article
C2 - 17511610
AN - SCOPUS:34247369794
VL - 72
SP - 445
EP - 451
JO - Biochemistry (Moscow)
JF - Biochemistry (Moscow)
SN - 0006-2979
IS - 4
ER -
ID: 99994930