Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Issledovanie éffektivnosti transliatsionnogo sopriazheniia v gibridnykh operonakh. / Mashko, S. V.; Lapidus, A. L.; Trukhan, M. E.; Stashchuk, N. A.; Debabov, V. G.
в: Molekulyarnaya Biologiya, Том 21, № 5, 09.1987, стр. 1310-1321.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Issledovanie éffektivnosti transliatsionnogo sopriazheniia v gibridnykh operonakh.
AU - Mashko, S. V.
AU - Lapidus, A. L.
AU - Trukhan, M. E.
AU - Stashchuk, N. A.
AU - Debabov, V. G.
PY - 1987/9
Y1 - 1987/9
N2 - The possibility of creating artificial overlappons was studied on the model of two genes, that coding for the N-terminal part of lambda cro protein and the cat of E. coli. To test the dependence of translational coupling efficiency on the intercistronic region a series of recombinant DNA molecules carrying different hybrid operons with partially overlapping genes was constructed. The translational efficiency of the distal to the promoter gene was shown to depend on the intercistronic region structure: overlapping of the AUG codon with the terminating one of the proximal gene in the UGAUG manner is optimal for the translational coupling, and the displacement of AUG at several nucleotides in both directions decreases the translational reinitiation efficiency for the ribosomes, that have synthesised the first gene product.
AB - The possibility of creating artificial overlappons was studied on the model of two genes, that coding for the N-terminal part of lambda cro protein and the cat of E. coli. To test the dependence of translational coupling efficiency on the intercistronic region a series of recombinant DNA molecules carrying different hybrid operons with partially overlapping genes was constructed. The translational efficiency of the distal to the promoter gene was shown to depend on the intercistronic region structure: overlapping of the AUG codon with the terminating one of the proximal gene in the UGAUG manner is optimal for the translational coupling, and the displacement of AUG at several nucleotides in both directions decreases the translational reinitiation efficiency for the ribosomes, that have synthesised the first gene product.
UR - http://www.scopus.com/inward/record.url?scp=0023414375&partnerID=8YFLogxK
M3 - статья
C2 - 2960884
AN - SCOPUS:0023414375
VL - 21
SP - 1310
EP - 1321
JO - МОЛЕКУЛЯРНАЯ БИОЛОГИЯ
JF - МОЛЕКУЛЯРНАЯ БИОЛОГИЯ
SN - 0026-8984
IS - 5
ER -
ID: 90141778