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Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography. / Dubrovskii, Ya A.; Gladilovich, V. D.; Krasnov, I. A.; Podolskaya, E. P.; Murashko, E. A.; Babakov, V. N.

в: Russian Journal of Bioorganic Chemistry, Том 38, № 1, 01.01.2012, стр. 41-45.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Dubrovskii, YA, Gladilovich, VD, Krasnov, IA, Podolskaya, EP, Murashko, EA & Babakov, VN 2012, 'Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography', Russian Journal of Bioorganic Chemistry, Том. 38, № 1, стр. 41-45. https://doi.org/10.1134/S1068162012010050

APA

Dubrovskii, Y. A., Gladilovich, V. D., Krasnov, I. A., Podolskaya, E. P., Murashko, E. A., & Babakov, V. N. (2012). Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography. Russian Journal of Bioorganic Chemistry, 38(1), 41-45. https://doi.org/10.1134/S1068162012010050

Vancouver

Dubrovskii YA, Gladilovich VD, Krasnov IA, Podolskaya EP, Murashko EA, Babakov VN. Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography. Russian Journal of Bioorganic Chemistry. 2012 Янв. 1;38(1):41-45. https://doi.org/10.1134/S1068162012010050

Author

Dubrovskii, Ya A. ; Gladilovich, V. D. ; Krasnov, I. A. ; Podolskaya, E. P. ; Murashko, E. A. ; Babakov, V. N. / Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography. в: Russian Journal of Bioorganic Chemistry. 2012 ; Том 38, № 1. стр. 41-45.

BibTeX

@article{f5e28baaee4c4d03a7093fc4beb8ef8a,
title = "Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography",
abstract = "Metal-affinity chromatography with Cu2+ containing sorbent was used for separation of globin peptides alkylated by sulfur mustard. It was shown that matrix-assisted laser desorption/ionization time-offlight mass spectrometry allowed isolating peptides alkylated by sulfur mustard (HD) at cysteine-126, -94 and glutamic acid-27 with MH+ of 1444.62, 1561.66, 1676.78 Da, respectively, from rat globin tryptic digest incubated with 60 μM of HD. An alkylated peptide with MH+ of 1444.63 Da was isolated from globin hydrolyzate incubated with 3 μM of HD.",
keywords = "Alkylation, Hemoglobin, MALDI, Sulfur mustard",
author = "Dubrovskii, {Ya A.} and Gladilovich, {V. D.} and Krasnov, {I. A.} and Podolskaya, {E. P.} and Murashko, {E. A.} and Babakov, {V. N.}",
year = "2012",
month = jan,
day = "1",
doi = "10.1134/S1068162012010050",
language = "English",
volume = "38",
pages = "41--45",
journal = "Russian Journal of Bioorganic Chemistry",
issn = "1068-1620",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "1",

}

RIS

TY - JOUR

T1 - Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography

AU - Dubrovskii, Ya A.

AU - Gladilovich, V. D.

AU - Krasnov, I. A.

AU - Podolskaya, E. P.

AU - Murashko, E. A.

AU - Babakov, V. N.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Metal-affinity chromatography with Cu2+ containing sorbent was used for separation of globin peptides alkylated by sulfur mustard. It was shown that matrix-assisted laser desorption/ionization time-offlight mass spectrometry allowed isolating peptides alkylated by sulfur mustard (HD) at cysteine-126, -94 and glutamic acid-27 with MH+ of 1444.62, 1561.66, 1676.78 Da, respectively, from rat globin tryptic digest incubated with 60 μM of HD. An alkylated peptide with MH+ of 1444.63 Da was isolated from globin hydrolyzate incubated with 3 μM of HD.

AB - Metal-affinity chromatography with Cu2+ containing sorbent was used for separation of globin peptides alkylated by sulfur mustard. It was shown that matrix-assisted laser desorption/ionization time-offlight mass spectrometry allowed isolating peptides alkylated by sulfur mustard (HD) at cysteine-126, -94 and glutamic acid-27 with MH+ of 1444.62, 1561.66, 1676.78 Da, respectively, from rat globin tryptic digest incubated with 60 μM of HD. An alkylated peptide with MH+ of 1444.63 Da was isolated from globin hydrolyzate incubated with 3 μM of HD.

KW - Alkylation

KW - Hemoglobin

KW - MALDI

KW - Sulfur mustard

UR - http://www.scopus.com/inward/record.url?scp=84861154097&partnerID=8YFLogxK

U2 - 10.1134/S1068162012010050

DO - 10.1134/S1068162012010050

M3 - Article

AN - SCOPUS:84861154097

VL - 38

SP - 41

EP - 45

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 1

ER -

ID: 36362513