We consider Euler-Bernoulli operators with real coefficients on the unit interval. We prove the following results: i) Ambarzumyan type theorem about the inverse problems for the Euler-Bernoulli operator. ii) The sharp asymptotics of eigenvalues for the Euler-Bernoulli operator when its coefficients converge to the constant function. iii) The sharp eigenvalue asymptotics both for the Euler-Bernoulli operator and fourth order operators (with complex coefficients) on the unit interval at high energy.