Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Intracellular immunoglobulins in Namalva and U266 cells cocultivated with mesenchymal stromal cells. / Ayzenshtadt, A. A.; Ivanova, N. A.; Bagaeva, V. V.; Smolyaninov, A. B.; Pinevich, A. A.; Samoylovich, M. P.; Klimovich, V. B.
в: Cell and Tissue Biology, Том 8, № 3, 01.01.2014, стр. 193-197.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Intracellular immunoglobulins in Namalva and U266 cells cocultivated with mesenchymal stromal cells
AU - Ayzenshtadt, A. A.
AU - Ivanova, N. A.
AU - Bagaeva, V. V.
AU - Smolyaninov, A. B.
AU - Pinevich, A. A.
AU - Samoylovich, M. P.
AU - Klimovich, V. B.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - The data concerning the influence of mesenchymal stromal cells (MSCs) on immunoglobulin (Ig) production are contradictory. Most results were obtained using MSC derived from bone marrow. The properties of MSCs obtained from other tissues are not well studied. In the present work, MSC cultures have been established from umbilical cord, adipose tissue, and bone marrow of healthy donors, as well as from bone marrow of patients with autoimmune diseases. MSCs from all these sources exhibited similar surface markers. We assayed the influence of MSC cocultivation at exponential or stationary growth phases on IgM content in Namalva and IgE content in U266 cells. Bone marrow MSCs from healthy donors did not affect IgM and IgE production. Proliferating MSCs from patients with Crohn's disease and multiple sclerosis stimulated Ig production. Exponentially growing MSCs derived from umbilical cord and adipose tissue also stimulated Ig synthesis. MSCs at stationary cultures enhanced IgM production in Namalva (cells) and suppressed IgE synthesis in U266 cells. Thus, MSCs from various tissues with common phenotypes differed in their capacity to modulate Ig production by B-lymphoid cells. The effect of MSCs depends on their growth stage and may be different for lymphoblastoid and myeloma cells.
AB - The data concerning the influence of mesenchymal stromal cells (MSCs) on immunoglobulin (Ig) production are contradictory. Most results were obtained using MSC derived from bone marrow. The properties of MSCs obtained from other tissues are not well studied. In the present work, MSC cultures have been established from umbilical cord, adipose tissue, and bone marrow of healthy donors, as well as from bone marrow of patients with autoimmune diseases. MSCs from all these sources exhibited similar surface markers. We assayed the influence of MSC cocultivation at exponential or stationary growth phases on IgM content in Namalva and IgE content in U266 cells. Bone marrow MSCs from healthy donors did not affect IgM and IgE production. Proliferating MSCs from patients with Crohn's disease and multiple sclerosis stimulated Ig production. Exponentially growing MSCs derived from umbilical cord and adipose tissue also stimulated Ig synthesis. MSCs at stationary cultures enhanced IgM production in Namalva (cells) and suppressed IgE synthesis in U266 cells. Thus, MSCs from various tissues with common phenotypes differed in their capacity to modulate Ig production by B-lymphoid cells. The effect of MSCs depends on their growth stage and may be different for lymphoblastoid and myeloma cells.
KW - immunoglobulins
KW - immunomodulation
KW - mesenchymal stromal cells
KW - Namalva
KW - U266
UR - http://www.scopus.com/inward/record.url?scp=84902441839&partnerID=8YFLogxK
U2 - 10.1134/S1990519X1403002X
DO - 10.1134/S1990519X1403002X
M3 - Article
AN - SCOPUS:84902441839
VL - 8
SP - 193
EP - 197
JO - Cell and Tissue Biology
JF - Cell and Tissue Biology
SN - 1990-519X
IS - 3
ER -
ID: 36329924