TY - JOUR

T1 - Intersectin is a negative regulator of dynamin recruitment to the synaptic endocytic zone in the central synapse

AU - Evergren, Emma

AU - Gad, Helge

AU - Walther, Kristin

AU - Sundborger, Anna

AU - Tomilin, Nikolay

AU - Shupliakov, Oleg

PY - 2007/1/10

Y1 - 2007/1/10

N2 - Intersectin is a multidomain dynamin-binding protein implicated in numerous functions in the nervous system, including synapse formation and endocytosis. Here,wedemonstrate that during neurotransmitter release in the central synapse, intersectin, like its binding partner dynamin, is redistributed from the synaptic vesicle pool to the periactive zone. Acute perturbation of the intersectin-dynamin interaction by microinjection of either intersectin antibodies or Src homology 3 (SH3) domains inhibited endocytosis at the fission step. Although the morphological effects induced by the different reagents were similar, antibody injections resulted in a dramatic increase in dynamin immunoreactivity around coated pits and at constricted necks, whereas synapses microinjected with the GST (glutathione S-transferase)-SH3C domain displayed reduced amounts of dynamin in the neck region. Our data suggest that intersectin controls the amount of dynamin released from the synaptic vesicle cluster to the periactive zone and that it may regulate fission of clathrin-coated intermediates.

AB - Intersectin is a multidomain dynamin-binding protein implicated in numerous functions in the nervous system, including synapse formation and endocytosis. Here,wedemonstrate that during neurotransmitter release in the central synapse, intersectin, like its binding partner dynamin, is redistributed from the synaptic vesicle pool to the periactive zone. Acute perturbation of the intersectin-dynamin interaction by microinjection of either intersectin antibodies or Src homology 3 (SH3) domains inhibited endocytosis at the fission step. Although the morphological effects induced by the different reagents were similar, antibody injections resulted in a dramatic increase in dynamin immunoreactivity around coated pits and at constricted necks, whereas synapses microinjected with the GST (glutathione S-transferase)-SH3C domain displayed reduced amounts of dynamin in the neck region. Our data suggest that intersectin controls the amount of dynamin released from the synaptic vesicle cluster to the periactive zone and that it may regulate fission of clathrin-coated intermediates.

KW - Glutamatergic neurons

KW - Periactive zone

KW - Presynaptic mechanisms

KW - Spinal cord

KW - Synaptic vesicle recycling

KW - Ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=33846254873&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.4683-06.2007

DO - 10.1523/JNEUROSCI.4683-06.2007

M3 - Article

C2 - 17215399

AN - SCOPUS:33846254873

VL - 27

SP - 379

EP - 390

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 2

ER -