Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Intersectin is a negative regulator of dynamin recruitment to the synaptic endocytic zone in the central synapse. / Evergren, Emma; Gad, Helge; Walther, Kristin; Sundborger, Anna; Tomilin, Nikolay; Shupliakov, Oleg.
в: Journal of Neuroscience, Том 27, № 2, 10.01.2007, стр. 379-390.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Intersectin is a negative regulator of dynamin recruitment to the synaptic endocytic zone in the central synapse
AU - Evergren, Emma
AU - Gad, Helge
AU - Walther, Kristin
AU - Sundborger, Anna
AU - Tomilin, Nikolay
AU - Shupliakov, Oleg
PY - 2007/1/10
Y1 - 2007/1/10
N2 - Intersectin is a multidomain dynamin-binding protein implicated in numerous functions in the nervous system, including synapse formation and endocytosis. Here,wedemonstrate that during neurotransmitter release in the central synapse, intersectin, like its binding partner dynamin, is redistributed from the synaptic vesicle pool to the periactive zone. Acute perturbation of the intersectin-dynamin interaction by microinjection of either intersectin antibodies or Src homology 3 (SH3) domains inhibited endocytosis at the fission step. Although the morphological effects induced by the different reagents were similar, antibody injections resulted in a dramatic increase in dynamin immunoreactivity around coated pits and at constricted necks, whereas synapses microinjected with the GST (glutathione S-transferase)-SH3C domain displayed reduced amounts of dynamin in the neck region. Our data suggest that intersectin controls the amount of dynamin released from the synaptic vesicle cluster to the periactive zone and that it may regulate fission of clathrin-coated intermediates.
AB - Intersectin is a multidomain dynamin-binding protein implicated in numerous functions in the nervous system, including synapse formation and endocytosis. Here,wedemonstrate that during neurotransmitter release in the central synapse, intersectin, like its binding partner dynamin, is redistributed from the synaptic vesicle pool to the periactive zone. Acute perturbation of the intersectin-dynamin interaction by microinjection of either intersectin antibodies or Src homology 3 (SH3) domains inhibited endocytosis at the fission step. Although the morphological effects induced by the different reagents were similar, antibody injections resulted in a dramatic increase in dynamin immunoreactivity around coated pits and at constricted necks, whereas synapses microinjected with the GST (glutathione S-transferase)-SH3C domain displayed reduced amounts of dynamin in the neck region. Our data suggest that intersectin controls the amount of dynamin released from the synaptic vesicle cluster to the periactive zone and that it may regulate fission of clathrin-coated intermediates.
KW - Glutamatergic neurons
KW - Periactive zone
KW - Presynaptic mechanisms
KW - Spinal cord
KW - Synaptic vesicle recycling
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=33846254873&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.4683-06.2007
DO - 10.1523/JNEUROSCI.4683-06.2007
M3 - Article
C2 - 17215399
AN - SCOPUS:33846254873
VL - 27
SP - 379
EP - 390
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 2
ER -
ID: 40832099