TY - JOUR

T1 - Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots

AU - Shumilina, Julia

AU - Kiryushkin, Alexey S.

AU - Frolova, Nadezhda

AU - Mashkina, Valeria

AU - Ilina, Elena L.

AU - Puchkova, Vera A.

AU - Danko, Katerina

AU - Silinskaya , Svetlana

AU - Serebryakov , Evgeny B.

AU - Soboleva , Alena

AU - Bilova , Tatiana

AU - Orlova , Anastasia

AU - Guseva, Elizaveta D.

AU - Repkin , Egor

AU - Pawlowski , Katharina

AU - Frolov, Andrej Frolov

AU - Demchenko , Kirill N.

N1 - Shumilina, J.; Kiryushkin, A.S.; Frolova, N.; Mashkina, V.; Ilina, E.L.; Puchkova, V.A.; Danko, K.; Silinskaya, S.; Serebryakov, E.B.; Soboleva, A.; Bilova, T.; Orlova, A.; Guseva, E.D.; Repkin, E.; Pawlowski, K.; Frolov, A.; Demchenko, K.N. Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots. Int. J. Mol. Sci. 2023, 24, 7654. https://doi.org/10.3390/ijms24087654

PY - 2023/4/21

Y1 - 2023/4/21

N2 - The main role of RALF small signaling peptides was reported to be the alkalization control of the apoplast for improvement of nutrient absorption; however, the exact function of individual RALF peptides such as RALF34 remains unknown. The Arabidopsis RALF34 (AtRALF34) peptide was proposed to be part of the gene regulatory network of lateral root initiation. Cucumber is an excellent model for studying a special form of lateral root initiation taking place in the meristem of the parental root. We attempted to elucidate the role of the regulatory pathway in which RALF34 is a participant using cucumber transgenic hairy roots overexpressing CsRALF34 for comprehensive, integrated metabolomics and proteomics studies, focusing on the analysis of stress response markers. CsRALF34 overexpression resulted in the inhibition of root growth and regulation of cell proliferation, specifically in blocking the G2/M transition in cucumber roots. Based on these results, we propose that CsRALF34 is not part of the gene regulatory networks involved in the early steps of lateral root initiation. Instead, we suggest that CsRALF34 modulates ROS homeostasis and triggers the controlled production of hydroxyl radicals in root cells, possibly associated with intracellular signal transduction. Altogether, our results support the role of RALF peptides as ROS regulators.

AB - The main role of RALF small signaling peptides was reported to be the alkalization control of the apoplast for improvement of nutrient absorption; however, the exact function of individual RALF peptides such as RALF34 remains unknown. The Arabidopsis RALF34 (AtRALF34) peptide was proposed to be part of the gene regulatory network of lateral root initiation. Cucumber is an excellent model for studying a special form of lateral root initiation taking place in the meristem of the parental root. We attempted to elucidate the role of the regulatory pathway in which RALF34 is a participant using cucumber transgenic hairy roots overexpressing CsRALF34 for comprehensive, integrated metabolomics and proteomics studies, focusing on the analysis of stress response markers. CsRALF34 overexpression resulted in the inhibition of root growth and regulation of cell proliferation, specifically in blocking the G2/M transition in cucumber roots. Based on these results, we propose that CsRALF34 is not part of the gene regulatory networks involved in the early steps of lateral root initiation. Instead, we suggest that CsRALF34 modulates ROS homeostasis and triggers the controlled production of hydroxyl radicals in root cells, possibly associated with intracellular signal transduction. Altogether, our results support the role of RALF peptides as ROS regulators.

KW - Cucumis sativus (cucumber)

KW - small signaling peptides

KW - RALF34

KW - root development

KW - OVEREXPRESSION

KW - hairy root transformation

KW - tandem mass spectrometry;

KW - proteomics

KW - metabolomics

KW - tandem mass spectrometry

KW - overexpression

UR - https://www.mendeley.com/catalogue/f4253465-7909-3a0c-891c-cb6b1fec446e/

U2 - https://doi.org/10.3390/ijms24087654

DO - https://doi.org/10.3390/ijms24087654

M3 - Article

VL - 24

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 8

M1 - 7654

ER -