TY - JOUR

T1 - Insect antimicrobial peptide complexes: naturally occurring solution of drug resistance problem

AU - Chernysh, S.I.

AU - Gordya, N.A.

AU - Suborova, T.N.

PY - 2014

Y1 - 2014

N2 - Last decades much attention has been paid to antimicrobial peptides (AMPs) as natural antibiotics presumably protected from resistance development in bacteria. However, experimental evolution studies revealed prompt resistance growth in bacteria to any individual AMP tested. Here we demonstrate that naturally occurring AMP complexes have clear advantage over individual peptide and small molecule antibiotics in respect of drug resistance development. As a model we have used AMP complexes of Calliphoridae flies living in extremely contaminated by bacteria environments. The AMP complex of blowfly Calliphora vicina was found to consist of three distinct families of cell membrane disrupting/permeabilizing peptides (defensins, cecropins and diptericins) and a family of DNA and protein synthesis damaging peptides (proline/arginine rich peptides). Resistance changes under long term selective pressure of the complex and reference antibiotics cefotaxime, meropenem and polymyxin B were tested using Escherichia coli, Kle

AB - Last decades much attention has been paid to antimicrobial peptides (AMPs) as natural antibiotics presumably protected from resistance development in bacteria. However, experimental evolution studies revealed prompt resistance growth in bacteria to any individual AMP tested. Here we demonstrate that naturally occurring AMP complexes have clear advantage over individual peptide and small molecule antibiotics in respect of drug resistance development. As a model we have used AMP complexes of Calliphoridae flies living in extremely contaminated by bacteria environments. The AMP complex of blowfly Calliphora vicina was found to consist of three distinct families of cell membrane disrupting/permeabilizing peptides (defensins, cecropins and diptericins) and a family of DNA and protein synthesis damaging peptides (proline/arginine rich peptides). Resistance changes under long term selective pressure of the complex and reference antibiotics cefotaxime, meropenem and polymyxin B were tested using Escherichia coli, Kle

KW - antimicrobial peptide complexes

KW - insects

KW - defensins

KW - diptericins

KW - proline-rich peptides

KW - bacteria

KW - antibiotic resistance

KW - drug resistance prevention

M3 - статья

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -