TY - JOUR

T1 - Inhibitors of the metabolism of arachidonic acid suppress Ca2+ responses induced by trifluoperazine in macrophages

AU - Milenina, L.S.

AU - Krutetskaya, Z.I.

AU - Naumova, A.A.

AU - Butov, S.N.

AU - Krutetskaya, N.I.

AU - Antonov, V.G.

N1 - Milenina, L.S., Krutetskaya, Z.I., Naumova, A.A. et al. Inhibitors of the Metabolism of Arachidonic Acid Suppress Ca2+ Responses Induced by Trifluoperazine in Macrophages. Cell Tiss. Biol. 12, 315–322 (2018). https://doi.org/10.1134/S1990519X18040065

PY - 2018

Y1 - 2018

N2 - The influence of the neuroleptic trifluoperazine on the intracellular concentration of Ca 2+ in macrophages of rats was studied using a Fura-2AM fluorescent Ca 2+ probe. It was found that trifluoperazine causes a dose-dependent increase in the intracellular Ca 2+ concentration associated with Ca 2+ mobilization from intracellular Ca 2+ stores and subsequent entry of Ca 2+ into peritoneal macrophages of rats. It was also shown that inhibitors of phospholipase A 2 (4-bromophenacyl bromide, prednisolone, and dexamethasone), cyclooxygenases (aspirin and indomethacin), and lipoxygenases (caffeic acid, zileuton, and baicalein) suppress Ca 2+ responses induced by trifluoperazine in macrophages. The data obtained indicate the participation of enzymes and/or products of the cascade of arachidonic acid metabolism in the influence of trifluoperazine on the intracellular concentration of Ca 2+ in peritoneal macrophages.

AB - The influence of the neuroleptic trifluoperazine on the intracellular concentration of Ca 2+ in macrophages of rats was studied using a Fura-2AM fluorescent Ca 2+ probe. It was found that trifluoperazine causes a dose-dependent increase in the intracellular Ca 2+ concentration associated with Ca 2+ mobilization from intracellular Ca 2+ stores and subsequent entry of Ca 2+ into peritoneal macrophages of rats. It was also shown that inhibitors of phospholipase A 2 (4-bromophenacyl bromide, prednisolone, and dexamethasone), cyclooxygenases (aspirin and indomethacin), and lipoxygenases (caffeic acid, zileuton, and baicalein) suppress Ca 2+ responses induced by trifluoperazine in macrophages. The data obtained indicate the participation of enzymes and/or products of the cascade of arachidonic acid metabolism in the influence of trifluoperazine on the intracellular concentration of Ca 2+ in peritoneal macrophages.

KW - arachidonic acid

KW - cyclooxygenase

KW - intracellular Ca concentration

KW - lipoxygenase

KW - peritoneal macrophages

KW - phospholipase A

KW - trifluoperazine

UR - http://www.scopus.com/inward/record.url?scp=85051539903&partnerID=8YFLogxK

U2 - 10.1134/S1990519X18040065

DO - 10.1134/S1990519X18040065

M3 - Article

VL - 12

SP - 315

EP - 322

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 4

ER -