Agro-industrial wastes such as spent grain and compost, amended with Azospirillum brasilense inoculation seeds before sowing were studied as to their effect on soil properties, plant growth parameters, seeds yield and nutrients composition of corn (Zea mays Var. 352.) under greenhouse conditions for four months. Azospirillum, one of the plant growth promoting bacteria (PGPB), has been recently used as a biofertilizer in soil to increase soil nitrogen fixation, phosphate availability and reducing soil salinity. Spent grain a by-product from beer industries, has a negative value for environmental quality due to its accumulation as an industrial waste. It has been recently applied as an environment-friendly organic waste increasing total organic carbon (TOC) in soil to improve its nutrient status. In this study, treatments of 1% (T1) and 2% (T2) of spent grain, 1% (C1) and 2% (C2) of compost and mix of both sources (C1T1), Azospirillum inoculation for soil (A1), Azospirillum with C1 (A1C1), Azospirillum with T1 (A1T1) were used and compared to the control. Treatments were mixed with calcareous soil; analysis showed varying nutrients composition with compost and spent grain 33% and 75%, of OM respectively, while spent grain had the highest nitrogen. The spent grain applied at 25.5gkg(-1)increased total nitrogen, phosphorus and potassium of corn. Azospirillum inoculation with organic wastes increased growth and seed yield significantly (P>0.05). The highest rate of spent grain with Azospirillum most effectively increased total organic carbon, macronutrients, growth parameters, and reduced soil Na+ concentration, being more effective than compost in improving properties of calcareous soil, and much non-expensive compared with compost or any chemical fertilizers.