Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Increased tRNA level in yeast cells with mutant translation termination factors eRF1 and eRF3. / Zhouravleva, G. A.; Moskalenko, S. E.; Chabelskaya, S. V.; Philippe, M.; Inge-Vechtomov, S. G.
в: Molecular Biology, Том 40, № 4, 07.2006, стр. 647-653.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Increased tRNA level in yeast cells with mutant translation termination factors eRF1 and eRF3
AU - Zhouravleva, G. A.
AU - Moskalenko, S. E.
AU - Chabelskaya, S. V.
AU - Philippe, M.
AU - Inge-Vechtomov, S. G.
N1 - Funding Information: ACKNOWLEDGMENTS This work was supported by the CRDF—Ministry of Education and Science Foundation (project no. ST-012), the Russian Foundation for Basic Research (project no. 03-04-48886), and the program Origin and Evolution of the Biosphere of the Presidium of the Russian Academy of Sciences. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/7
Y1 - 2006/7
N2 - We have earlier characterized Saccharomyces cerevisiae strains with mutations of essential SUP45 and SUP35, which code for translation termination factors eRF1 and eRF3, respectively. In this work, the sup45 and sup35 nonsense mutants were compared with respect to the levels of eight tRNAs: tRNA Tyr, tRNAGln, tRNATrp, tRNALeu, tRNAArg (described as potential suppressor tRNAs), tRNA Pro, tRNAHis, and tRNAGly. The mutants did not display a selective increase in tRNAs, capable of a noncanonical read-through at stop codons. Most of the mutations increased the level of all tRNAs under study. The mechanisms providing for the viability of the sup45 and sup35 nonsense mutants are discussed.
AB - We have earlier characterized Saccharomyces cerevisiae strains with mutations of essential SUP45 and SUP35, which code for translation termination factors eRF1 and eRF3, respectively. In this work, the sup45 and sup35 nonsense mutants were compared with respect to the levels of eight tRNAs: tRNA Tyr, tRNAGln, tRNATrp, tRNALeu, tRNAArg (described as potential suppressor tRNAs), tRNA Pro, tRNAHis, and tRNAGly. The mutants did not display a selective increase in tRNAs, capable of a noncanonical read-through at stop codons. Most of the mutations increased the level of all tRNAs under study. The mechanisms providing for the viability of the sup45 and sup35 nonsense mutants are discussed.
KW - eRF1
KW - eRF3
KW - Saccharomyces cerevisiae
KW - Translation termination
KW - tRNA
UR - http://www.scopus.com/inward/record.url?scp=33747124360&partnerID=8YFLogxK
U2 - 10.1134/S0026893306040170
DO - 10.1134/S0026893306040170
M3 - Article
AN - SCOPUS:33747124360
VL - 40
SP - 647
EP - 653
JO - Molecular Biology
JF - Molecular Biology
SN - 0026-8933
IS - 4
ER -
ID: 70378862