Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Increased paracellular permeability of tumor-adjacent areas in 1,2-dimethylhydrazine-induced colon carcinogenesis in rats. / Bekusova, Viktoria V; Falchuk, Evgeny L; Okorokova, Larisa S; Kruglova, Natalia M; Nozdrachev, Alexander D; Markov, Alexander G.
в: Cancer Biology and Medicine, Том 15, № 3, 01.08.2018, стр. 251-259.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Increased paracellular permeability of tumor-adjacent areas in 1,2-dimethylhydrazine-induced colon carcinogenesis in rats
AU - Bekusova, Viktoria V
AU - Falchuk, Evgeny L
AU - Okorokova, Larisa S
AU - Kruglova, Natalia M
AU - Nozdrachev, Alexander D
AU - Markov, Alexander G
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Objective: The morphology and functions of the proximal and distal large intestine are not the same. The incidence of colorectal cancer in these regions is also different, as tumors more often appear in the descending colon than in the ascending colon. Inflammatory bowel disease and colorectal cancer can increase transepithelial permeability, which is a sign of reduced intestinal barrier function. However, there is not enough evidence to establish a connection between the difference in colorectal cancer incidence in the proximal and distal colon and intestinal permeability or the effects of carcinogenesis on the barrier properties in various areas of the colon. The aim of the study was to assess the permeability of different segments of the large intestine according to a developed mapping methodology in healthy rats and rats with 1,2-dimethylhydrazine (DMH)-induced colon adenocarcinoma.Methods: The short circuit current, the transepithelial electrical resistance and the paracellular permeability to fluorescein of large intestine wall of male Wistar rats were examined in the Ussing chambers. The optical density of the solution from the serosa side to assess the concentration of the diffused fluorescein from mucosa to serosa was analyzed by spectrophotometry. The morphometric and histological studies were performed by optical microscopy.Results: Rats with DMH-induced colon adenocarcinomas showed elevated transepithelial electrical resistance in the areas of neoplasm development. In contrast, there was no change in the electrophysiological properties of tumor adjacent areas, however, the paracellular permeability of these areas to fluorescein was increased compared to the control rats and was characterized by sharply reduced barrier function.Conclusions: The barrier properties of the colon vary depending on tumor location. The tumors were less permeable than the intact intestinal wall and probably have a negative influence on tumor-adjacent tissues by disrupting their barrier function.
AB - Objective: The morphology and functions of the proximal and distal large intestine are not the same. The incidence of colorectal cancer in these regions is also different, as tumors more often appear in the descending colon than in the ascending colon. Inflammatory bowel disease and colorectal cancer can increase transepithelial permeability, which is a sign of reduced intestinal barrier function. However, there is not enough evidence to establish a connection between the difference in colorectal cancer incidence in the proximal and distal colon and intestinal permeability or the effects of carcinogenesis on the barrier properties in various areas of the colon. The aim of the study was to assess the permeability of different segments of the large intestine according to a developed mapping methodology in healthy rats and rats with 1,2-dimethylhydrazine (DMH)-induced colon adenocarcinoma.Methods: The short circuit current, the transepithelial electrical resistance and the paracellular permeability to fluorescein of large intestine wall of male Wistar rats were examined in the Ussing chambers. The optical density of the solution from the serosa side to assess the concentration of the diffused fluorescein from mucosa to serosa was analyzed by spectrophotometry. The morphometric and histological studies were performed by optical microscopy.Results: Rats with DMH-induced colon adenocarcinomas showed elevated transepithelial electrical resistance in the areas of neoplasm development. In contrast, there was no change in the electrophysiological properties of tumor adjacent areas, however, the paracellular permeability of these areas to fluorescein was increased compared to the control rats and was characterized by sharply reduced barrier function.Conclusions: The barrier properties of the colon vary depending on tumor location. The tumors were less permeable than the intact intestinal wall and probably have a negative influence on tumor-adjacent tissues by disrupting their barrier function.
KW - крыса, 1,2-диметилгидразин, рак толстой кишки, камера Уссинга, ток короткого замыкания, трансэпителиальное сопротивление, проницаемость
KW - Rat; 1,2-dimethylhydrazine; colorectal cancer; Ussing chamber; short circuit current; transepithelial electrical resistance; intestinal permeability
KW - Rat
KW - Short circuit current
KW - Colorectal cancer
KW - Intestinal permeability
KW - Ussing chamber
KW - Transepithelial electrical resistance
KW - 1,2-dimethylhydrazine
UR - http://www.scopus.com/inward/record.url?scp=85055428944&partnerID=8YFLogxK
U2 - 10.20892/j.issn.2095-3941.2018.0016
DO - 10.20892/j.issn.2095-3941.2018.0016
M3 - Article
C2 - 30197792
VL - 15
SP - 251
EP - 259
JO - Cancer Biology and Medicine
JF - Cancer Biology and Medicine
SN - 2095-3941
IS - 3
ER -
ID: 33266456